+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic and functional characterization of in vivo tissue engineered smooth muscle from normal and pathological bladders

Phenotypic and functional characterization of in vivo tissue engineered smooth muscle from normal and pathological bladders

Journal of Urology 168(4 Pt 2): 1853-7; Discussion 1858

Purpose: The engineering of bladder tissue involves obtaining a biopsy from a host, expanding the cells, seeding them onto a matrix and implanting the cell-matrix composite back into the host. Clinically, cells used for these techniques may be harvested from abnormal bladders. It is not known whether abnormal bladder cells may be engineered into functionally normal tissue. We investigated the phenotypic and functional characteristics of tissue engineered bladder smooth muscle derived from patients with functionally normal bladders and functionally abnormal exstrophic and neuropathic bladders. Materials and Methods: Human smooth muscle cells derived from functionally normal bladders, exstrophic bladders and neurogenic bladders were grown, expanded and seeded onto polymer scaffolds. Sixteen cell seeded scaffolds were analyzed in vitro and 40 cell seeded scaffolds were implanted in athymic mice. The tissue engineered constructs were retrieved and analyzed at 2 weeks and 2 months. The scaffolds were evaluated immunocytochemically, histologically, with organ bath studies and with Western blot analyses. Results: Human bladder cells showed similar expression of smooth muscle marker proteins (alpha-actin and myosin) in vitro and after 2 months in vivo, regardless of their origin. All scaffolds showed similar muscle formation in vivo. The cell seeded scaffolds demonstrated the typical "contraction-relaxation" response to supramaximal electrical field and carbachol stimulation. There were no statistical differences among the experimental groups (normal, exstrophic, neurogenic). Conclusions: Tissue engineered muscle from normal and diseased bladders retain their phenotype in vitro and after implantation in vivo. The cells exhibited the same degree of contractility to electrical and chemical stimulation regardless of their origin. These results suggest that there are no phenotypic or functional differences between muscle cells obtained from urodynamically normal or pathological bladders, and that bladder muscle cells, regardless of their origin, may have the potential to be engineered into normal bladder tissues.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 046973843

Download citation: RISBibTeXText

PMID: 12352375

Related references

Phenotypic and Functional Characterization of In Vivo Tissue Engineered Smooth Muscle From Normal and Pathological Bladders. The Journal of Urology 168(4): 1853-1858, 2002

Phenotypic and functional characterization of in vivo tissue engineered smooth muscle from normal and pathological bladders. International Braz J Urol 28(5): 486-487, 2005

Comparison of in vivo contractility of tissue engineered smooth muscle from normal and pathological bladders using different scaffolds. Journal of Urology 167(4 Suppl.): 58, 2002

Does the Mesenchymal Stem Cell Source Influence Smooth Muscle Regeneration in Tissue-Engineered Urinary Bladders?. Cell Transplantation 26(11): 1780-1791, 2017

Ex vivo functional evaluation of isolated strips in BAMG tissue-engineered bladders. International Journal of Artificial Organs 32(3): 159-165, 2009

Ex vivo Functional Evaluation of Isolated Strips in Bamg Tissue-Engineered Bladders. The International Journal of Artificial Organs 32(3): 159-165, 2009

Vascular smooth muscle enhances functionality of tissue-engineered blood vessels in vivo. Journal of Vascular Surgery 53(2): 426-434, 2011

Functional recovering of human smooth muscle cell in a tissue engineered vascular media. Molecular Biology of the Cell 12(Suppl.): 511a, 2001

Correlation between the distribution of smooth muscle or non muscle myosins and alpha-smooth muscle actin in normal and pathological soft tissues. Cell Motility and the Cytoskeleton 11(4): 260-274, 1988

Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury. Tissue Engineering. Part a 18(11-12): 1213-1228, 2012

Functional characterization of serum- and growth factor-induced phenotypic changes in intact bovine tracheal smooth muscle. British Journal of Pharmacology 137(4): 459-466, 2002

Biomimetic control of vascular smooth muscle cell morphology and phenotype for functional tissue-engineered small-diameter blood vessels. Journal of Biomedical Materials Research. Part a 88(4): 1104-1121, 2009

Length-tension relations of smooth muscle from normal and denervated rat urinary bladders. Acta Physiologica Scandinavica 112(4): 443-447, 1981

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair. Journal of Visualized Experiments 2016(116):, 2016

Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells. Journal of Materials Science. Materials in Medicine 23(4): 1109-1117, 2012