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Phenotypic and genotypic alterations associated with the attenuation of a Theileria annulata vaccine cell line from Turkey



Phenotypic and genotypic alterations associated with the attenuation of a Theileria annulata vaccine cell line from Turkey



Vaccine 16(6): 569-575



Attenuated vaccines, produced by prolonged in vitro culture of the macroschizont stage of the life-cycle, are the main method of controlling Theileria annulata infections. Little is known about the mechanism(s) of attenuation. Here we present data from a Turkish cell line demonstrating that attenuation is associated with reduced ability to differentiate into microschizonts and a reduction in matrix metalloproteinase activity. We also show that attenuation results in a change in the structure of the parasite population. Using the technique of differential mRNA display, we demonstrate that gene expression profiles differ between non-attenuated and attenuated macroschizont infected leucocytes. One differentially expressed gene is of parasite origin. These data are discussed in the context of a multifactorial model for virulence.

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Accession: 046973889

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PMID: 9569467

DOI: 10.1016/s0264-410x(97)84509-4


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