+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic and genotypic characterization of Mannheimia (Pasteurella) haemolytica-like strains isolated from diseased animals in Denmark



Phenotypic and genotypic characterization of Mannheimia (Pasteurella) haemolytica-like strains isolated from diseased animals in Denmark



Veterinary Microbiology 84(1-2): 103-114



Trehalose-negative strains of the Pasteurella haemolytica complex have recently been transferred to a new genus, Mannheimia. This genus presently consists of five named species: M. haemolytica, M. glucosida, M. granulomatis, M. ruminalis and M. varigena. The purpose of this study was to investigate the occurrence of these species and lesions associated with these isolates in Denmark. In all 106 M. haemolytica-like strains isolated from pathological material from cattle, sheep, pigs and hares submitted to the Danish Veterinary Laboratory between 1994 and 1998 were investigated. Phenotypic characterization and ribotyping were used for identification in addition to sequencing of the 16S rRNA genes for selected strains. The species allocation was determined by comparison to results from a previous polyphasic taxonomic study. Seventy-one percent of the strains belonged to M. haemolytica, 18% to M. varigena and 8% to unnamed groups within the genus Mannheimia. Single isolates identified as M. glucosida and P. trehalosi, respectively, were detected. Two isolates belonged to M. granulomatis. Forty-three percent of the strains belonged to serotype 1, 41% were untypeable, while the rest belonged to serotypes 2, 7, 9, and 16. The present investigation also showed that a simplified phenotypic characterization using Diatabs Diagnostic Tablets (Rosco, Denmark) represents a useful method for obtaining a quick and reliable species identification. Finally, the investigation confirmed that serotyping does not represent a reliable method for species identification. The heterogeneity of species associated with bovine "pasteurellosis" should be considered in future studies to improve our understanding of the pathogenesis of pneumonic disease.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046973903

Download citation: RISBibTeXText

PMID: 11731163


Related references

Phenotypic and genotypic characterization of Mannheimia (Pasteurella) haemolytica-like strains isolated from diseased animals in Denmark. Veterinary Microbiology 84(1/2): 103-114, 2002

Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. International Journal of Systematic Bacteriology 49 Pt 1: 67-86, 1999

Minimum inhibitory concentrations (MIC) of frequently used chemotherapeutics present in agents pathogenic to animals as ascertained by multicentric systems in Germany. Part III: Pasteurella and Mannheimia spp. (Pasteurella multocida, Mannheimia haemolytica). Praktische Tierarzt 82(3): 214-224, 2001

Serotypes A1 and A2 of Mannheimia haemolytica are susceptible to genotypic, capsular and phenotypic variations in contrast to T3 and T4 serotypes of Bibersteinia (Pasteurella) trehalosi. Fems Microbiology Letters 280(1): 42-49, 2008

Serotypes of Mannheimia haemolytica and Pasteurella trehalosi strains isolated from nasal cavity of healthy small ruminants appear to be less cytotoxic than those isolated from clinical pasteurellosis. Revue de Medecine Veterinaire 156(11): 574-577, 2005

Mannheimia haemolytica and Pasteurella multocida occurrence in healthy FT Ocorrencia de Mannheimia haemolytica e de Pasteurella multocida em ovinos sadios e com enfermidade respiratoria. 2007

Serological and molecular comparison of Mannheimia haemolytica and Pasteurella trehalosi strains isolated from wild and domestic ruminants in the French Alps. Veterinary Journal 171(3): 545-550, 2006

Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Mannheimia haemolytica strains isolated from cattle, sheep and goats. Veterinarni Medicina 49(3): 65-69, 2004

Cross protection of a Mannheimia haemolytica A1 Lkt-/Pasteurella multocida ΔhyaE bovine respiratory disease vaccine against experimental challenge with Mannheimia haemolytica A6 in calves. Vaccine 30(13): 2320-2328, 2012

Mosaic structure and molecular evolution of the leukotoxin operon (lktCABD) in Mannheimia (Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi. Journal of Bacteriology 184(1): 266-277, 2002

Sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompA) of Mannheimia(Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi. Journal of Bacteriology 186(17): 5741-5752, 2004

Phenotypic characterisation of Australian sheep and cattle isolates of Mannheimia haemolytica, Mannheimia granulomatis and Mannheimia varigena. Australian Veterinary Journal 80(1-2): 87-91, January-February, 2002

Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1. Journal of Antimicrobial ChemoTherapy 48(5): 631-640, 2001

Taxonomic relationships of the haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. International Journal of Systematic Bacteriology 49(1): 67-86, 1999

DNA characterization of Mannheimia haemolytica A2, A7, A9 and Pasteurella trehalosi. Journal of Animal and Veterinary Advances 4(2): 210-213, 2005