+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups



Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups



Journal of Clinical Microbiology 38(9): 3179-3186



Phenotypic and genotypic diversity of the flagellin gene (fliC) of Clostridium difficile was studied in 47 isolates from various origins belonging to the serogroups A, B, C, D, F, G, H, I, K, X, and S3. Electron microscopy revealed 17 nonflagellated strains and 30 flagellated strains. PCR and reverse transcription-PCR demonstrated that the flagellin gene was present in all strains and that the fliC gene was expressed in both flagellated and nonflagellated strains. Southern blotting showed the presence of only one copy of the gene and three different hybridization patterns. DNA sequence analysis of fliC from the strains belonging to serogroups C, D, and X, representative of each profile, disclosed great variability in the central domain, whereas the N- and C-terminal domains were conserved. The variability of the flagellin gene fliC was further studied in the isolates by PCR-restriction fragment length polymorphism (RFLP) analysis. Nine different RFLP groups were identified (I to IX), among which three (I, VII, and VIII) corresponded to numerous serogroups whereas the six others (II, III, IV, V, VI, and IX) belonged to a single serogroup. Flagellin gene RFLP analysis could constitute an additional typing method employable in conjunction with other typing methods currently available.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046973920

Download citation: RISBibTeXText

PMID: 10970353


Related references

Clostridium difficile flagellin FliC: Evaluation as adjuvant and use in a mucosal vaccine against Clostridium difficile. Plos one 12(11): E0187212, 2017

The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection. Plos one 9(5): E96876, 2014

Phenotypic and genotypic analysis of Clostridium difficile isolates: a single-center study. Journal of Clinical Microbiology 52(12): 4260-4266, 2014

Prospective study of Clostridium difficile infections in Europe with phenotypic and genotypic characterisation of the isolates. Clinical Microbiology and Infection 13(11): 1048-1057, 2007

Protection against Clostridium difficile infection in a hamster model by oral vaccination using flagellin FliC-loaded pectin beads. Vaccine 36(40): 6017-6021, 2018

Molecular characterization of fliD gene encoding flagellar cap and its expression among Clostridium difficile isolates from different serogroups. Journal of Clinical Microbiology 39(3): 1178-1183, 2001

Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. Journal of Veterinary Internal Medicine 16(5): 533-540, 2002

Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. Journal of Veterinary Internal Medicine 17(2): 123; Author Reply 123, 2003

A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. Journal of Clinical Microbiology 36(8): 2240-2247, 1998

Genotypic characterization of Enterobacter sakazakii isolates by PFGE, BOX-PCR and sequencing of the fliC gene. Journal of Applied Microbiology 104(1): 26-34, 2008

Identification of Clostridium difficile: evaluation of genotypic, phenotypic and proteomic methods. British Journal of Biomedical Science 70(3): 128-129, 2013

A Clostridium difficile gene encoding flagellin. Microbiology 146: 957-966, 2000

Genotypic and phenotypic analysis of Clostridium difficile correlated with previous antibiotic exposure. Microbial Drug Resistance 12(1): 23-28, 2006

Characterization of an aberrant Clostridium difficile strain with apparent genotypic/phenotypic discordance. Abstracts of the General Meeting of the American Society for Microbiology 94: 31, 1994

Molecular characterization of Clostridium difficile flagellin gene. Abstracts of the General Meeting of the American Society for Microbiology 99: 30, 1999