+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic and molecular characterization of clinical isolates of Mycobacterium elephantis from human specimens



Phenotypic and molecular characterization of clinical isolates of Mycobacterium elephantis from human specimens



Journal of Clinical Microbiology 40(4): 1230-1236



Eleven strains of a rapidly growing mycobacterium were isolated from patient specimens originating from various regions of the province of Ontario, Canada, over a 2-year period. Unique high-performance liquid chromatography (HPLC) and PCR-restriction enzyme pattern analysis (PRA) profiles initially suggested a new Mycobacterium species, while sequencing of the 16S rRNA gene revealed a sequence match with Mycobacterium sp. strain MCRO 17 (GenBank accession no. X93028), an isolate determined to be unique which is to date uncharacterized, and also a close similarity to M. elephantis (GenBank accession no. AJ010747), with six base pair variations. A complete biochemical profile of these isolates revealed a species of mycobacteria with phenotypic characteristics similar to those of M. flavescens. HPLC, PRA, and 16S rRNA sequencing of strain M. elephantis DSM 44368(T) and result comparisons with the clinical isolates revealed that these strains were in fact M. elephantis, a newly described species isolated from an elephant. All strains were isolated from human samples, 10 from sputum and 1 from an axillary lymph node.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 046973947

Download citation: RISBibTeXText

PMID: 11923337


Related references

Molecular characterization of class 3 integrons from Delftia spp.v. Journal of Bacteriology 189(17): 6276-6283, 2007

Phenotypic and molecular characterization of human monoclonal TCR gamma/delta T-cell lines from jejunum and colon of healthy individuals. Scandinavian Journal of Immunology 38(3): 247-253, 1993

Phenotypic and molecular characteristics of Escherichia coli isolated from aquatic environment of Bangladesh. Microbiology and Immunology 50(5): 359-370, 2006

Mycobacterium culture preparation with tri-sodium phosphate. Zentralblatt für Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie Virusforschung und Parasitologie. Originale 170(8): 608-615, 1958

Phenotypic and molecular characterization of Lactococcus lactis from milk and plants. Journal of Applied Microbiology 101(2): 396-405, 2006

Study of pesticides in waters from a Chalk catchment, Cambridgeshire. Pesticide science2(1): 15-33, 1991

Phenotypic & genetic characterization of Bacillus cereus isolated from the acute diarrhoeal patients. Indian Journal of Medical Research 133: 88-95, 2011

Characterization of rotavirus strains from day care centers: pre- and post-rotavirus vaccine era. Jornal de Pediatria 86(2): 155-158, 2010

Molecular and phenotypic characterization of Listeria monocytogenes from U.S. Department of Agriculture Food Safety and Inspection Service surveillance of ready-to-eat foods and processing facilities. Journal of Food Protection 73(5): 861-869, 2010

Taxonomic value of microbial oxidases and cytochrome oxidases. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 156: 1904-1907, 1962

Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus from surgical site infections. Surgical Infections 14(2): 196-202, 2013

Association between elimination disorders and abusive maternal attitudes. Journal of Forensic and Legal Medicine 40: 22-27, 2016

Identification of Mycobacterium bovis Isolates by a multiplex PCR. Brazilian Journal of Microbiology 40(2): 231-233, 2009

Molecular and phenotypic characterization of Vibrio aestuarianus, a pathogen of the cultured tongue sole, Cynoglossus semilaevis Günther. Journal of Fish Diseases 34(1): 57-64, 2011

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae). Insect Biochemistry and Molecular Biology 36(12): 921-933, 2006