+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic and morphological characterization of neuroblastoma cells constitutively expressing B-myb

Phenotypic and morphological characterization of neuroblastoma cells constitutively expressing B-myb

Journal of Neuro-Oncology 31(1-2): 107-114

B-myb gene is expressed in neuroblastoma cells and down-regulated during differentiation. We used B-myb-transfected LAN-5 cells, which constitutively express high level of B-myb, to detect changes at phenotypic and morphological levels in basal and differentiation conditions. Our results demonstrate that the overexpression of B-myb markedly affects the cytoskeletal composition, the pattern of neurotransmitter enzymes and the extracellular matrix expression. In general, B-myb transfected neuroblastoma cells show a broad potentiality without a direction toward a specific neuroectodermal differentiation pathway. On the other hand, we confirm inhibition of the neuronal differentiation upon retinoic acid (RA) treatment of B-myb transfected cells. Furthermore, the ultrastructural analyses are supportive of a change in the metabolism in B-myb transfected cell treated with RA. Our data suggest that B-myb expression is compatible with an early phase of differentiation of neuroectodermal cells, but must be down-regulated for the completion of the differentiative programme.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 046973955

Download citation: RISBibTeXText

PMID: 9049836

DOI: 10.1023/a:1005749802210

Related references

Coordinate regulation of oncogenes suppressor genes and cell cycle genes in human neuroblastoma cell lines sensitive to retinoic acid and its disruption in nb cells constitutively expressing igf ii. Biomedicine & Pharmacotherapy 46(5-7): 289, 1992

Morphological but not cholinergic phenotypic differentiation in NGF-treated NNR5 cells expressing TrkA receptors. Society for Neuroscience Abstracts 25(1-2): 2024, 1999

Phenotypic and Morphological Properties of Germinal Center Dark Zone Cxcl12-Expressing Reticular Cells. Journal of Immunology 195(10): 4781-4791, 2015

Osteoclastogenic activity and RANKL expression are inhibited in osteoblastic cells expressing constitutively active Gα(12) or constitutively active RhoA. Journal of Cellular Biochemistry 111(6): 1531-1536, 2010

Phenotypic and functional characterization of CD4 T cells expressing killer Ig-like receptors. Journal of Immunology 173(11): 6719-6726, 2004

Phenotypic and molecular characterization of proliferating and differentiated GnRH-expressing GnV-3 cells. MolecularandCellularEndocrinology332(1-2):97, 2011

Phenotypic characterization of GPR120-expressing cells in the interstitial tissue of pancreas. Tissue and Cell 45(6): 421-427, 2013

PC12nnr5 cells expressing TrkA receptors undergo morphological but not cholinergic phenotypic differentiation in response to nerve growth factor. Journal of Neurochemistry 80(3): 501-511, 2002

Frequency and characterization of phenotypic Ig heavy chain allelically included IgM-expressing B cells in mice. Journal of Immunology 164(2): 893-899, 2000

Detection by in situ hybridization and phenotypic characterization of cells expressing IL-6 mRNA in human stimulated blood. Journal of Immunology 144(4): 1317-1322, 1990

Phenotypic and morphological characterization of the differentiated NTera2 cells. Society for Neuroscience Abstracts 24(1-2): 1527, 1998

Phenotypic characterization of neurotensin messenger RNA-expressing cells in the neuroleptic-treated rat striatum: a detailed cellular co-expression study. Neuroscience 76(3): 763-774, 1997

Characterization of transgenic rats constitutively expressing vitamin D-24-hydroxylase gene. Biochemical & Biophysical Research Communications 297(5): 1332-1338, 2002

Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization. Journal of Allergy and Clinical Immunology 139(1): 335-346.E3, 2017

The potency of inhibiting Notch - cleavage is increased in cells stably or constitutively expressing Notch versus transiently expressing Notch. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 295 17, 2003