+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phenotypic change of human cultured meningioma cells



Phenotypic change of human cultured meningioma cells



Journal of Neuro-Oncology 49(1): 9-17



One objection to using cell cultures for studying the proliferation of tumors is the potential for phenotypic changes that may occur in vitro. Here, we compared the antigen pattern expression of cultured meningioma cells with that of the primary tumor. Cell cultures established from 9 intracranial meningiomas and deparaffinized sections of the resected tumors were analyzed for immunophenotyping with the following antibodies: vimentin, cytokeratin, epithelial membrane antigen, S-100, neuron-specific enolase, synaptophisin, factor VIII-related antigen, CD4, CD31, CD34, CD45RB, CD68-PGM1, CD68-KP, and myeloid/histiocyte antigen (MAC387). Overall, the cultured meningioma cells retained the main feature of the primary tumor, being positive both for mesenchymal antigens and for epithelial antigens. Interestingly, the cultured meningioma cells abundantly expressed the CD68 antigens at early passage. The CD68 antigens, which are normally found on hematopoietic cells like macrophages and monocytes, were not detectable on meningioma cells in situ. Our results show that phenotypic changes on human meningioma cells may occur in vitro. This phenomenon suggests caution when transposing the in vitro results to the in vivo condition.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 046973970

Download citation: RISBibTeXText

PMID: 11131990

DOI: 10.1023/a:1006436903976


Related references

An ultrastructural study of cultured human meningioma cells. Acta Neuropathologica 46(1-2): 11-16, 1979

Phenotypic change of adrenomedullin receptor components in cultured rat vascular smooth muscle cells. Japanese Journal of Pharmacology 85(Suppl. 1): 137P, 2001

Phenotypic change of endothelin receptor subtype in cultured rat vascular smooth muscle cells. Endocrinology 134(1): 222-228, 1994

Stimulation of IL-6 secretion in cultured human meningioma cells by IL-1-beta and IL-4. Journal of Endocrinology 137(Suppl. ): P106, 1993

Epidermal growth factor--receptors on cultured human meningioma cells. Acta Neurochirurgica 83(1-2): 62-66, 1986

Inhibition of the growth of cultured human meningioma cells by recombinant interferon-alpha. European Journal of Cancer 27(4): 416-419, 1991

The regulatory effects of protein kinase C on the proliferation of cultured human low-passage meningioma cells. Journal of Tongji Medical University 20(3): 217-219, 2000

Somatostatin inhibits the activity of adenylate cyclase in cultured human meningioma cells and stimulates their growth. Journal of Clinical Endocrinology and Metabolism 74(3): 543-547, 1992

Subcellular distribution and photocytotoxicity of aluminium phthalocyanines and haematoporphyrin derivative in cultured human meningioma cells. British Journal of Neurosurgery 10(1): 51-57, 1996

Immunohistochemical study of anaplastic meningioma with special reference to the phenotypic change of intermediate filament protein. Annals of Diagnostic Pathology 7(4): 214-222, 2003

"Inverse signaling" of the transmembrane chemokine CXCL16 contributes to proliferative and anti-apoptotic effects in cultured human meningioma cells. Cell Communication and Signaling 14(1): 26, 2016

Interleukin 1 induces acute phenotypic change in human glomerular mesangial cells shape change cytoskeletal reorganization and shift in cytoskeletal protein isotypes. Clinical Research 37(2): 487A, 1989

The temporal and molecular phenotypic expression of cultured human bone cells. Calcified Tissue International 64(Suppl. 1): S107, 1999

Phenotypic and functional characterization of in vitro cultured human mast cells. Cytometry. Part B Clinical Cytometry 92(5): 348-354, 2017

Phenotypic evaluation of cultured human mast and basophilic cells and of normal human skin mast cells. Archives Of Dermatological Research. 286(7): 380-385, 1994