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Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography



Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography



Journal of Chromatography. a 905(1-2): 151-155



High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation.

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Accession: 047052582

Download citation: RISBibTeXText

PMID: 11206781

DOI: 10.1016/s0021-9673(00)00987-0


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