+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Progress towards the construction of a transmissible gastroenteritis coronavirus self-replicating RNA using a two-layer expression system

Progress towards the construction of a transmissible gastroenteritis coronavirus self-replicating RNA using a two-layer expression system

Advances in Experimental Medicine and Biology 440: 319-325

Three transmissible gastroenteritis coronavirus (TGEV) defective interfering RNAs of 21, 10.6 and 9.7 kb (DI-A, DI-B and DI-C, respectively) were isolated. Dilution experiments showed that the largest DI RNA, DI-A, is a self-replicating RNA (replicon), and thus codes for a functional RNA polymerase and all the necessary replication signals. In order to engineer a cDNA encoding the RNA replicon a strategy based on the cloning of DI-C cDNA, followed by the insertion of the sequences required to complete the DI-A sequence has been developed. A cDNA complementary to DI-C RNA was cloned under the control of the CMV promoter (pDI-C-CMV) and rescued with a helper virus. In the ORF 1a of polymerase gene pDI-C-CMV contained a 10 kb deletion and in ORF 1b a 1.1 kb deletion. The consensus sequence corresponding to the deleted regions was cloned, and the deletions in pDI-C-CMV were replaced to yield a complete cDNA clone of DI-A, pDI-A-21-CMV, containing a full-length TGEV polymerase, driven by a CMV promoter. Expression of a functional TGEV polymerase is being investigated.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 047095522

Download citation: RISBibTeXText

PMID: 9782299

Related references

Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus. Veterinary Immunology & Immunopathology 48(1-2): 35-54, 1995

Expression of the porcine transmissible gastroenteritis coronavirus M protein. Advances in Experimental Medicine and Biology 276: 121-126, 1990

Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants. Virology 249(2): 352-358, 1998

Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus. American journal of veterinary research 54(2): 254-259, 1993

Transcription regulatory sequences and mRNA expression levels in the coronavirus transmissible gastroenteritis virus. Journal of Virology 76(3): 1293-1308, 2002

In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes. Journal of General Virology 83(Pt 3): 567-579, 2002

Isolation of coronavirus from the lungs of piglets of herds infected with transmissible gastroenteritis coronavirus. Trudy Vsesoyuznogo Instituta Eksperimental' noi Veterinarii 53: 114-120, 1981

Oral immunization of sows against transmissible gastroenteritis using the transmission gastroenteritis coronavirus strain 188 sg. Annales de Zootechnie 34(3): 371-372, 1985

Expression of transcriptional units using transmissible gastroenteritis coronavirus derived minigenomes and full-length cDNA clones. Advances in Experimental Medicine and Biology 494: 447-451, 2001

Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd. Archives of Virology 145(6): 1133-1147, 2000

Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits. Msphere 4(2):, 2019

Identification of phenanthroindolizines and phenanthroquinolizidines as novel potent anti-coronaviral agents for porcine enteropathogenic coronavirus transmissible gastroenteritis virus and human severe acute respiratory syndrome coronavirus. Antiviral Research 88(2): 160-168, 2010

Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity. Journal of Virology 70(8): 5634-5637, 1996

Transmissible gastroenteritis coronavirus carrier sow. Advances in Experimental Medicine and Biology 440: 641-647, 1998

Characterization of transmissible gastroenteritis coronavirus S protein expression products in avirulent S. typhimurium delta cya delta crp: persistence, stability and immune response in swine. VeterinaryMicrobiology48(1-2):87, 1996