+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Promoter elements required for phloem-specific gene expression from the RTBV promoter in rice

Promoter elements required for phloem-specific gene expression from the RTBV promoter in rice

Plant Journal 12(5): 1179-1188

Previous studies indicated that a DNA fragment comprising nucleotides (nt) -164 to +45 of the RTBV promoter is sufficient to drive phloem-specific expression of a reporter gene in transgenic rice plants. In addition, two potential cis elements, Box I (nt -3 to +5) and Box II (nt -53 to -39) were identified by DNA-protein interaction assays. In this study, the results of further in vivo studies involving mutagenesis of selected DNA sequences and analysis of expression of a reporter gene in transgenic rice plants revealed that, in addition to Box I and Box II, other elements are required for phloem-specific gene expression, among which are a direct repeat (ASL Box, nt -98 to -79) and a GATA motif (nt -143 to -135). All the these elements bind rice nuclear factors specifically, and mutations of the elements were identified that resulted in loss-of-competition in electrophoretic mobility shift assays. A DNA fragment comprising nt -164 to -32, which contains Box II, the ASL Box and the GATA motif, conferred phloem-specific reporter gene expression independent of Box I when fused to a heterologous CaMV 35S minimal promoter and introduced to transgenic rice plants. Studies that introduced point mutations suggested that in the context of the -103 to +45 promoter fragment, Box II and the ASL Box act synergistically to confer tissue-specific gene expression. Similar studies in the -164 to +45 promoter fragment indicated that the -164 to -103 region, which includes the GATA motif, contains sequences that are functionally redundant with those in the -103 to -32 region, including the ASL Box and Box II. It is concluded that these regions act additively to direct phloem-specific gene expression.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 047099874

Download citation: RISBibTeXText

PMID: 9418055

Related references

Cloning of a wheat puroindoline gene promoter by IPCR and analysis of promoter regions required for tissue-specific expression in transgenic rice seeds. Plant Molecular Biology 39(6): 1101-1112, 1999

Functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcription. Plant Molecular Biology 32(6): 1019-1027, 1996

Promoter Elements Required for Developmental Expression of the Maize Adh1 Gene in Transgenic Rice. The Plant Cell 6(6): 799-810, 1994

Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice. Plant Cell 6(6): 799-810, 1994

Promoter elements required for hormonal and tissue specific expression of the rat anf gene in the heart. Journal of Cellular Biochemistry Suppl. (13 Part E): 182, 1989

RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Molecular Biology 62(3): 397-408, 2006

Structure and promoter analysis of Math3 gene, a mouse homolog of Drosophila proneural gene atonal. Neural-specific expression by dual promoter elements. Journal of Biological Chemistry 273(11): 6327-6333, 1998

Characterization of proximal promoter elements required for cell-specific expression of the human neurotensin gene. Gastroenterology 108(4 Suppl. ): A964, 1995

A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific gene expression in transgenic plants. Plant Biotechnology Journal 5(5): 664-674, 2007

The rice OsLTP6 gene promoter directs anther-specific expression by a combination of positive and negative regulatory elements. Planta 238(5): 845-857, 2013

Two of three promoter elements identified in a rice gene for proliferating cell nuclear antigen are essential for meristematic tissue-specific expression. Plant Journal 7(6): 877-886, 1995

Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone H1oo, Nucleoplasmin 2, and Zygote Arrest 1) are required for oocyte-specific gene expression in the mouse. Molecular Reproduction and Development 75(7): 1104-1108, 2008

Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene. Reproduction 146(5): 501-516, 2013

Characterization of promoter elements required for cell-specific expression of the neurotensin/neuromedin N gene in a human endocrine cell line. Molecular and Cellular Biology 15(7): 3870-3881, 1995

Cell specific expression of mouse albumin promoter evidence for cell specific dna elements within the proximal promoter region and cis acting dna elements upstream of minus 160. Journal of Biological Chemistry 264(16): 9171-9179, 1989