+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation

Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation

Letters in Applied Microbiology 23(6): 403-406

Glucoamylases produced by Aspergillus niger grown on wheat brain in solid cultures were purified. Four different forms, GA I, GA I', GA II and GA III, were found having apparent molecular weights of 112,000, 104,000, and 74,000 and 61,000 Da respectively. The enzymes are glycoproteins with a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2+ were stimulatory. The Km values for the degradation of starch and maltose were 3.5 and 7.8 mg ml-1, respectively.

(PDF emailed within 0-6 h: $19.90)

Accession: 047134453

Download citation: RISBibTeXText

PMID: 8987901

DOI: 10.1111/j.1472-765x.1996.tb01346.x

Related references

Purification and biochemical characterization of an Aspergillus niger phytase produced by solid-state fermentation using triticale residues as substrate. Biotechnology Reports 17: 49-54, 2018

Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation. Letters in Applied Microbiology 39(1): 13-18, 2004

Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger. Biotechnology Techniques 9(12): 901-906, 1995

Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate. Bioresource Technology. 65(1-2): 83-85, Y-., 1998

Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation. Applied Biochemistry and Biotechnology 128(2): 131-140, 2006

Glucoamylase production by Aspergillus niger in solid state fermentation with paddy husk as support. Journal of Food Science and Technology Mysore 38(4): 334-338, 2001

Effect of yeast extract on glucoamylase synthesis by Aspergillus niger in solid state fermentation. Indian Journal of Microbiology 35(4): 335-338, 1995

Characterization of β -Glucosidase Produced by Aspergillus niger under Solid-State Fermentation and Partially Purified Using MANAE-Agarose. Biotechnology Research International 2014: 317092-317092, 2014

X-ray diffraction studies on solid state fermentation for the production of glucoamylase using Aspergillus niger NCIM 1248. Journal of Scientific and Industrial Research 57(10/11): 583-586, 1998

Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching. Bioprocess and Biosystems Engineering 32(6): 819-824, 2009

Purification and characterization of acid-stable protopectinase produced by Aspergillus awamori in solid-state fermentation. Bioscience, Biotechnology, and Biochemistry 64(7): 1337-1344, 2000

Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme. Journal of Microbiology 52(5): 389-398, 2014

Purification and partial characterization of cellulase-free xylanase produced in solid-state and submerged fermentation by Aspergillus tamarii. Advances in Food Sciences 22(1-2): 31-37, 2000

Multi-enzyme product with glucoamylase, proteolytic and xylanase activities and method for producing same by solid state fermentation of wheat bran with Aspergillus niger. Official Gazette of the United States Patent & Trademark Office Patents 1277(4), Dec 23, 2003

Optimization of extraction and purification of glucoamylase produced byAspergillus awamoriin solid-state fermentation. Biotechnology and Bioprocess Engineering 14(1): 60-66, 2009