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Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation



Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation



Letters in Applied Microbiology 23(6): 403-406



Glucoamylases produced by Aspergillus niger grown on wheat brain in solid cultures were purified. Four different forms, GA I, GA I', GA II and GA III, were found having apparent molecular weights of 112,000, 104,000, and 74,000 and 61,000 Da respectively. The enzymes are glycoproteins with a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2+ were stimulatory. The Km values for the degradation of starch and maltose were 3.5 and 7.8 mg ml-1, respectively.

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Accession: 047134453

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PMID: 8987901

DOI: 10.1111/j.1472-765x.1996.tb01346.x


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