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Rapid and reversible uncoupling of the hepatic alpha 1-adrenergic receptor and guanine-nucleotide-binding protein by phorbol 12,13-dibutyrate


Rapid and reversible uncoupling of the hepatic alpha 1-adrenergic receptor and guanine-nucleotide-binding protein by phorbol 12,13-dibutyrate



Biochimica et Biophysica Acta 1176(3): 339-342



ISSN/ISBN: 0006-3002

PMID: 8386000

Phorbol 12,13-dibutyrate (PDBU), 1 microgram/ml for 2 min, abolished alpha 1-adrenergic receptor (AR)-mediated signal transduction in rat hepatocytes and converted 100% of alpha 1-ARs to a low-affinity state, similar to effects of a non-hydrolyzable GTP analog. Reversal of PDBU inhibition restored high-affinity alpha 1-ARs towards control values. The rapid uncoupling of the alpha 1-AR and guanine-nucleotide-binding protein by PDBU, and reversibility associated with reactivation of alpha 1-adrenergic signaling, identify this as an important inhibitory locus for PDBU.

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Accession: 047170130

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Rapid and reversible uncoupling of the hepatic α1-adrenergic receptor and guanine-nucleotide-binding protein by phorbol 12,13-dibutyrate. Biochimica et Biophysica Acta - Molecular Cell Research 1176(3): 339-342, 1993

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