+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system



Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system



Journal of Virological Methods 78(1-2): 105-116



An improved quantitative polymerase chain reaction (qPCR) method based on a combination of real-time detection and the 5'-3' nuclease activity of the Taq DNA polymerase was developed to quantify the provirus load of feline immunodeficiency virus (FIV), a lentivirus of veterinary importance and an animal model for AIDS research. Two fluorogenic probes were designed to detect FIV provirus in genomic DNA of peripheral lymphocytes and tissues infected with different FIV subtypes. The most sensitive assay can detect one copy of FIV provirus. The assay showed excellent precision within-run and between-runs. Comparison of the TaqMan system with a conventional seminested PCR assay revealed a comparable detection limit and good correlation. Furthermore the design of the two probes allowed the detection of various FIV isolates of clade A and B.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 047171356

Download citation: RISBibTeXText

PMID: 10204701

DOI: 10.1016/s0166-0934(98)00166-9


Related references

Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqManReg. fluorogenic real-time detection system. Journal of Virological Methods 78(1/2): 105-116, 1999

Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction. Journal of Virological Methods 130(1-2): 124-132, 2005

Detection of foot and mouth disease virus by real-time, fluorogenic TaqMan reverse transcription polymerase chain reaction assay. Chinese Journal of Veterinary Science 25(2): 116-119, 2005

Development of TaqMan real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus. Journal of Virological Methods 234: 132-136, 2016

Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays. Virologica Sinica 27(5): 273-277, 2012

Detection of feline immunodeficiency provirus by seminested polymerase chain reaction. Folia Microbiologica 45(2): 161-165, 2000

TaqMan real-time polymerase chain reaction assay for rapid detection of Flavobacterium columnare. Aquaculture research 38(5): 508-517, 2007

Detection of feline immunodeficiency provirus in domestic cats by polymerase chain reaction. Pesquisa Veterinaria Brasileira 20(1): 20-25, 2000

Detection of dengue virus by real-time polymerase chain reaction with TaqMan MGB probe. Chinese Journal of Zoonoses 21(8): 716-720, 2005

Detection of dengue virus from Aedes albopictus by TaqMan MGB real-time polymerase chain reaction. Zhongguo Meijieshengwuxue Ji Kongzhi Zazhi il; 17(2): 86-89, 2006

The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction. Veterinary Microbiology 38(1-2): 11-21, 1993

Real-time fluorogenic reverse transcription polymerase chain reaction assay for the specific detection of Bagaza virus. Journal of Veterinary Diagnostic Investigation 24(5): 959-963, 2012

The development of a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using TaqMan technology for the pan detection of bluetongue virus (BTV). Journal of Virological Methods 245: 35-39, 2017

Development of a TaqMan-based real-time reverse transcription polymerase chain reaction assay for the detection of encephalomyocarditis virus. Journal of Virological Methods 207: 60-65, 2014

Comparative evaluation of a novel TaqMan real-time reverse transcription-polymerase chain reaction assay for hepatitis A virus detection. Journal of International Medical Research 41(2): 427-434, 2013