+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Recombinant vaccinia viruses which express MHV-JHM proteins: protective immune response and the influence of vaccination on coronavirus-induced encephalomyelitis



Recombinant vaccinia viruses which express MHV-JHM proteins: protective immune response and the influence of vaccination on coronavirus-induced encephalomyelitis



Advances in Experimental Medicine and Biology 342: 401-406



Vaccinia-virus (VV) recombinants encoding either the nucleocapsid (N) or the spike (S) protein of MHV-JHM were constructed to study the role of the immune response against defined coronavirus antigens. For the S-protein, a fusogenic (Sfus+) or non fusogenic variant (Sfus-) of the gene was inserted into the VV genome. A strong protection against acute encephalomyelitis (AE) was mediated in Lewis rats which were immunized by VV-Sfus+ and challenged with an otherwise lethal dose of MHV-JHM before the induction of S-specific IgG antibodies. By contrast, a VV recombinant encoding a variant non fusogenic S-protein or the N-protein was not capable conferring protection. In addition, we demonstrated that MHV-JHM S-specific IgG antibodies elicited before MHV-JHM challenge modulated the disease process, changing it from an acute disease to subacute demyelinating encephalomyelitis (SDE).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 047190293

Download citation: RISBibTeXText

PMID: 8209761


Related references

The analysis of immune effector mechanisms using recombinant vaccinia viruses that express their own cytokines. Journal of Cellular Biochemistry Suppl. (15 Part F): 147, 1991

Protective immunity to vaccinia virus induced by vaccination with multiple recombinant outer membrane proteins of intracellular and extracellular virions. Journal of Virology 78(19): 10230-7, 2004

Evaluation of the protective efficacy of recombinant vaccinia viruses and adenoviruses that express respiratory syncytial virus glycoproteins. Brown, F , Et Al (Ed ) Vaccines (Cold Spring Harbor), 90 Modern Approaches to New Vaccines Including Prevention Of Aids; Seventh Meeting, September 1989 Xxv+502p Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, Usa Illus Paper 79-84, 1990

Induction of protective immunity in animals vaccinated with recombinant vaccinia viruses that express PreM and E glycoproteins of Japanese encephalitis virus. Journal of Virology 64(6): 2788-2795, 1990

Active vaccination with vaccinia virus A33 protects mice against lethal vaccinia and ectromelia viruses but not against cowpoxvirus; elucidation of the specific adaptive immune response. Virology Journal 10: 229, 2013

Protective and disease-enhancing immune responses induced by recombinant modified vaccinia Ankara (MVA) expressing respiratory syncytial virus proteins. Vaccine 23(2): 215-221, 2004

Vaccination with recombinant vaccinia viruses expressing ICP27 induces protective immunity against herpes simplex virus through CD4+ Th1+ T cells. Journal of Virology 69(8): 4711-4716, 1995

Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61(12): 3855-3861, 1987

Immune response of mice infected with recombinant vaccinia viruses carrying the HIV gag gene. Microbiology and Immunology 35(10): 849-861, 1991

Recombinant N and S1 proteins of infectious bronchitis virus (IBV) induced anti-viral protective immune response. IV International symposium on avian corona and pneumovirus infections, Rauischholzhausen, Germany, 20-23 June 2004: 237-243, 2004

Intrathecal humoral immune response in coronavirus induced encephalomyelitis of Lewis and BN rats. Advances in Experimental Medicine and Biology 218: 373-381, 1987

Coronavirus jhm induced demyelinating encephalomyelitis in rats analysis of the intrathecal immune response. Journal of Neuroimmunology 16(1): 46-47, 1987

Coronavirus-JHM-induced demyelinating encephalomyelitis in rats. Analysis of the intrathecal immune response. Annals of the new York Academy of Sciences 540: 663-664, 1988

Hepatitis B virus proteins expressed by recombinant vaccinia viruses: Influence of preS2 sequence on expression surface and nucleocapsid proteins in human diploid cells. Archives of Virology 134(1-2): 1-15, 1994