+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regulation of coronavirus RNA transcription is likely mediated by protein-RNA interactions

Regulation of coronavirus RNA transcription is likely mediated by protein-RNA interactions

Advances in Experimental Medicine and Biology 380: 515-521

Coronavirus mRNA transcription was thought to be regulated by the interaction between the leader RNA and the intergenic (IG) sequence, probably involving direct RNA-RNA interactions between complementary sequences. In this study, we found that a 9-nucleotide sequence immediately downstream of the leader RNA up-regulated mRNA transcription and that a particular strain of mouse hepatitis virus (MHV) lacking this 9-nucleotide transcribed subgenomic mRNA species containing unusually heterogeneous leader-fusion sites. These results suggest that the sequence complementarity between the leader and IG is not necessarily required for mRNA transcription. UV cross-linking experiments using cytoplasmic extracts of uninfected cells and the IG sequence showed that three different cellular proteins bound to IG of the template RNA. Deletion analyses and site-directed mutagenesis of IG further demonstrated a correlation between protein-binding and transcription efficiency, suggesting that these RNA-binding proteins are involved in the regulation of coronavirus mRNA transcription. We propose that coronavirus transcription is regulated by RNA-protein and protein-protein interactions.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 047212781

Download citation: RISBibTeXText

PMID: 8830534

Related references

RNA-protein interactions in the regulation of coronavirus RNA replication and transcription. Biological Chemistry 378(6): 477-481, 1997

RNA-RNA and RNA-protein interactions in coronavirus replication and transcription. Rna Biology 8(2): 237-248, 2011

Coronavirus-induced ER stress response and its involvement in regulation of coronavirus-host interactions. Virus Research 194: 110-123, 2014

Coronavirus transcription mediated by sequences flanking the transcription consensus sequence. Virology 217(1): 311-322, 1996

Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription. Journal of Virology 62(11): 4280-4287, 1988

Up-regulation of tyrosine hydroxylase gene transcription by tetradecanoylphorbol acetate is mediated by the transcription factors Ets-like protein-1 (Elk-1) and Egr-1. Journal of Neurochemistry 97(1): 92, 2006

Host protein interactions with the 3' end of bovine coronavirus RNA and the requirement of the poly(A) tail for coronavirus defective genome replication. Journal of Virology 74(11): 5053-5065, 2000

Regulation of coronavirus transcription: viral and cellular proteins interacting with transcription-regulating sequences. Advances in Experimental Medicine and Biology 581: 31-35, 2006

Repression of activator-mediated transcription by herpes simplex virus ICP4 via a mechanism involving interactions with the basal transcription factors TATA-binding protein and TFIIB. Molecular and Cellular Biology 15(7): 3618-3626, 1995

Target specificity and protein-protein interactions in the regulation of transcription by hox gene products. Journal of Cellular Biochemistry Suppl. 0(17 Part A): 213, 1993

Regulation of FoxO transcription factors by acetylation and protein-protein interactions. Biochimica et Biophysica Acta 1813(11): 1954-1960, 2011

Regulation of the maize anthocyanin pathway involves protein/protein interactions between transcription factors. Journal of Cellular Biochemistry Suppl. 0(19A): 136, 1995

Role of protein--protein interactions in the regulation of transcription by trp repressor investigated by fluorescence spectroscopy. Biochemistry 31(13): 3429-3441, 1992

Functional switches in transcription regulation; molecular mimicry and plasticity in protein-protein interactions. Biochemistry 43(25): 7983-7991, 2004

Detection of regulatory circuits by integrating the cellular networks of protein-protein interactions and transcription regulation. Nucleic Acids Research 31(20): 6053-6061, 2003