+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler

Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler

Methods in Molecular Biology 197: 129-137

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 047241915

Download citation: RISBibTeXText

PMID: 12013791

DOI: 10.1385/1-59259-284-8:129

Related references

Quantitative analysis of copy number variants based on real-time LightCycler PCR. Current Protocols in Human Genetics 80: Unit 7.21., 2014

The Alterations in Mitochondrial DNA Copy Number and Nuclear-Encoded Mitochondrial Genes in Rat Brain Structures after Cocaine Self-Administration. Molecular Neurobiology 54(9): 7460-7470, 2017

Development of a quantitative PCR (TaqMan) assay for relative mitochondrial DNA copy number and the common mitochondrial DNA deletion in the rat. Environmental and Molecular Mutagenesis 44(4): 313-320, 2004

Nuclear and mitochondrial DNAs microsatellite instability and mitochondrial DNA copy number in adenocarcinoma and squamous cell carcinoma of lung: a pilot study. Apmis 123(12): 1048-1054, 2015

Two nuclear genes mrs3 and mrs4 which can suppress a mitochondrial splice defect when present on a high copy number plasmid belong to the mitochondrial carrier family. Yeast 6(SPEC ISSUE): S530, 1990

Precise determination of mitochondrial DNA copy number in human skeletal and cardiac muscle by a PCR-based assay: lack of change of copy number with age. Nucleic Acids Research 31(11): E61, 2003

LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270(1): 119-133, 1 December, 2002

Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth. Genetics and Molecular Research 14(1): 1662-1670, 2015

PCR-Based Analysis of Mitochondrial DNA Copy Number, Mitochondrial DNA Damage, and Nuclear DNA Damage. Current Protocols in Toxicology 67: 20.11.1-20.11.25, 2016

Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number. Current Genetics 12(7): 503-510, 1987

Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies. Forensic Science International 139(2-3): 141-149, 2004

TaqMan real-time PCR quantification strategy of CYP2D6 gene copy number for the LightCycler 2.0. Clinica Chimica Acta; International Journal of Clinical Chemistry 403(1-2): 207-211, 2009

Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A. Nucleic Acids Research 40(20): 10124-10138, 2012