+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Retroviral vector-mediated gene transfer into umbilical cord blood-derived megakaryocyte and platelet progenitors



Retroviral vector-mediated gene transfer into umbilical cord blood-derived megakaryocyte and platelet progenitors



Biology of Blood and Marrow Transplantation 5(4): 215-221



Optimizing platelet engraftment following hematopoietic stem cell transplantation is essential for minimizing transplant-related morbidity, particularly following umbilical cord blood transplantation (UCBT), where platelet engraftment frequently takes >60 days. One strategy for optimizing platelet engraftment following UCBT is to study or alter the genetic program of megakaryocyte/platelet (Mk/plt) progenitors. Retroviral vector gene transfer has previously proven useful for studying the biology of hematopoietic stem cells; however, procedures for transducing UCB cells of the Mk/plt lineage with retroviral vectors have not been described. We report here that Mk/plt progenitors generated from UCB progenitors can be efficiently transduced with retroviral vectors. Transduced Mk/plt cells were identified and quantitated by expression of a vector transgene encoding a truncated version of the human nerve growth factor receptor (NGFR). Vector-mediated NGFR expression could be readily detected in Mk/plt progenitors defined by immunophenotypic, morphologic, and functional criteria. In addition, NGFR expression persisted in mature anucleate platelets generated from the transduced Mk/plt progenitors. These methods may be useful for introducing genetic elements into Mk/plt progenitors to study various aspects of platelet development and biology and for marking ex vivo expanded Mk/plt progenitors to determine their contribution to engraftment.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 047264180

Download citation: RISBibTeXText

PMID: 10465101

DOI: 10.1053/bbmt.1999.v5.pm10465101


Related references

Retroviral vector-mediated gene transfer into umbilical cord blood CD34brCD38-CD33- cells. Experimental Hematology 27(8): 1244-1254, 1999

Efficient retroviral mediated transfer of the glucocerebrosidase gene in CD34+ enriched umbilical cord blood human hematopoietic progenitors. Experimental Hematology 23(14): 1628-1632, 1995

Ex vivo expansion and retroviral vector transduction of umbilical cord blood platelet progenitors. Blood 90(10 Suppl. 1 Part 1): 537A, 1997

Comparison of retroviral-mediated gene transfer into cultured human CD34+ hematopoietic progenitor cells derived from peripheral blood, bone marrow, and fetal umbilical cord blood. Biology of Blood and Marrow Transplantation 3(5): 273-281, 1997

Transduction of CD34-enriched human peripheral and umbilical cord blood progenitors using a retroviral vector with the Fanconi anemia group C gene. Journal of Investigative Medicine 43(4): 379-385, 1995

Retroviral vector gene transfer into primitive umbilical cord blood cells. Blood 90(10 Suppl. 1 Part 2): 412B, 1997

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor (BDNF) tohuman umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) promotescrush-injured rat sciatic nerve regeneration. Neuroscience Letters 643: 111-120, 2017

Retroviral mediated gene transduction of recombinant human erythropoietin receptor into daughter progenitors derived from single CD34+++ cord blood cells changes the differentiation profile of daughter progenitors. Blood 88(10 Suppl. 1 Part 1-2): 44A, 1996

Multiparameter-fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells. Experimental Hematology 26(4): 288-298, 1998

Multiparameter fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells. Blood 88(10 Suppl. 1 Part 1-2): 435A, 1996

Retroviral-mediated gene transfer into hematopoietic stem progenitor cells from fresh and cryopreserved umbilical cord blood. Gluckman, E, Coulombel, L, INSERM Colloquium; Ontogeny of hematopoiesis: Aplastic anemia, Colloque INSERM; Ontogenie de l'hematopoiese: Aplasie medullaire 115-119, 1995

Gene transfer into human umbilical cord blood-derived CD34+ cells by particle-mediated gene transfer. Gene Therapy. 5(5): 692-699,, 1998

Retroviral mediated gene transfer of the enhanced green fluorescent protein gene into human umbilical cord blood cells with NOD/SCID mouse engrafting potential. Experimental Hematology 26(8): 687, 1998

Construction and transduction of human interleukin -9 receptor gene into human cord blood progenitors using retroviral-mediated gene transfer. Experimental Hematology 22(8): 681, 1994

Enhancing effects of co-transduction of cDNAs for c-kit and steel factor by retroviral mediated gene transfer on growth of erythroid and multipotential progenitors in CD34++ cord blood cells. Blood 90(10 Suppl. 1 Part 2): 414B, 1997