+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Sphingosine-1-phosphate mobilizes intracellular calcium and activates transcription factor NF-kappa B in U937 cells



Sphingosine-1-phosphate mobilizes intracellular calcium and activates transcription factor NF-kappa B in U937 cells



Biochemical and Biophysical Research Communications 234(1): 121-124



Sphingosine-1-phosphate (SPP), a metabolite of sphingolipids, has been implicated as a second messenger in cell growth regulation and signal transduction via calcium mobilization from internal stores. This study shows that SPP mobilizes intracellular calcium in U937 cells and demonstrates for the first time the ability of SPP to activate the transcription factor NF-kappa B in these cells. Furthermore, calcium release from the internal stores by thapsigargin (TG), an inhibitor of the endoplasmic reticulum Ca2+ pump, was associated with activation of NF-kappa B. Moreover, we have shown that while an intracellular calcium chelator BAPTA/AM was able to inhibit both SPP- and TG-induced NF-kappa B activation, it had no effect on TNF-induced NF-kappa B activation. In addition, SPP-induced NF-kappa B activation was blocked both by cyclosporin A, known to inhibit calcineurin phosphatase activity, and by the antioxidant butylated hydroxyanisole. These observations suggest that intracellular calcium mobilization is required for SPP-induced NF-kappa B activation, which may involve calcineurin- and redox-dependent mechanisms.

(PDF emailed within 0-6 h: $19.90)

Accession: 047414730

Download citation: RISBibTeXText

PMID: 9168973

DOI: 10.1006/bbrc.1997.6598


Related references

Sphingosine 1-phosphate mobilizes sequestered calcium, activates calcium entry, and stimulates deoxyribonucleic acid synthesis in thyroid FRTL-5 cells. Endocrinology 138(10): 4049-4057, 1997

Sphingosine 1-phosphate activates nuclear factor-kappa B through Edg receptors. Activation through Edg-3 and Edg-5, but not Edg-1, in human embryonic kidney 293 cells. Journal of Biological Chemistry 276(52): 48733-9, 2001

Enhancement of intracellular sphingosine-1-phosphate production by inositol 1,4,5-trisphosphate-evoked calcium mobilisation in HEK-293 cells: endogenous sphingosine-1-phosphate as a modulator of the calcium response. Cellular Signalling 17(7): 827-836, 2005

Linoleic acid activates nuclear transcription factor-kappa B (NF-kappa B) and induces NF-kappa B-dependent transcription in cultured endothelial cells. American journal of clinical nutrition 63(3): 322-328, 1996

Endothelin 1 activates phospholipase c and mobilizes calcium from extra cellular and intracellular pools in osteoblastic cells. American Journal of Physiology 257(6 PART 1): E797-E803, 1989

Urokinase activates calcium-dependent potassium channels in U937 cells via calcium release from intracellular stores. European Journal of Biochemistry 265(1): 264-272, 1999

Ceramide 1-phosphate increases intracellular free calcium concentrations in thyroid FRTL-5 cells: evidence for an effect mediated by inositol 1,4,5-trisphosphate and intracellular sphingosine 1-phosphate. Biochemical Journal 370(Pt 1): 111-119, 2002

Angiotensin II mobilizes intracellular calcium and activates pannexin-1 channels in rat carotid body type II cells via AT1 receptors. Journal of Physiology 592(21): 4747-4762, 2015

Sphingosine mobilizes intracellular calcium in human neutrophils. Cell Calcium 14(6): 493-505, 1993

Extracellular adenosine triphosphate activates phospholipase C and mobilizes intracellular calcium in primary cultures of sheep anterior pituitary cells. Febs Letters 243(2): 333-336, 1989

3,4-Methylenedioxymethamphetamine activates nuclear factor-kappaB, increases intracellular calcium, and modulates gene transcription in rat heart cells. Cardiovascular Toxicology 5(3): 301-310, 2005

The neuropeptide substance P activates transcription factor NF-kappa B and kappa B-dependent gene expression in human astrocytoma cells. Journal of Immunology 159(10): 4952-4958, 1997

Neuropeptide y mobilizes intracellular calcium and increases inositol phosphate production in human erythroleukemia cells. Biochemical & Biophysical Research Communications 165(3): 1138-1144, 1989

Raf-1 protein kinase activates the NF-kappa B transcription factor by dissociating the cytoplasmic NF-kappa B-I kappa B complex. Proceedings of the National Academy of Sciences of the United States of America 90(20): 9247-9251, 1993

Sphingosine 1-phosphate activates Erk-1/-2 by transactivating epidermal growth factor receptor in rat-2 cells. Iubmb Life 50(2): 119-124, 2001