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Substrate specificity of the human coronavirus 229E 3C-like proteinase



Substrate specificity of the human coronavirus 229E 3C-like proteinase



Advances in Experimental Medicine and Biology 440: 115-120



Coronavirus gene expression involves proteolytic processing of the gene 1-encoded polyproteins and a key enzyme in this process is the virus-encoded 3C-like proteinase. In this study, we describe the biosynthesis of the human coronavirus 229E 3C-like proteinase in Escherichia coli and the substrate specificity of the purified protein. Using immunofluorescence microscopy, we have also investigated the subcellular localization of the 3C-like proteinase and have found a punctate, perinuclear distribution of the proteinase in virus-infected cells.

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Accession: 047474109

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PMID: 9782272


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