EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Synthetic peptide and template-assembled synthetic protein models of the hen egg white lysozyme 87-97 helix: importance of a protein-like framework for conformational stability in a short peptide sequence


Synthetic peptide and template-assembled synthetic protein models of the hen egg white lysozyme 87-97 helix: importance of a protein-like framework for conformational stability in a short peptide sequence



Biopolymers 33(3): 389-400



ISSN/ISBN: 0006-3525

PMID: 8461450

DOI: 10.1002/bip.360330307

In the native structure of hen egg white lysozyme (HEL), the amino acid sequence 87-97 (HEL 87-97) forms an amphiphilic helix, with hydrophilic residues in the sequence directed toward the solvent. A synthetic version of the HEL 87-97 sequence (with the cysteine corresponding to position 94 of HEL replaced by alanine) displays conformational features in solution typical of an unordered structure as judged by CD. However, various modifications in the sequence result in increased helix-forming potential of the HEL 87-97 analogues. Further stabilization of the helical conformation in the most helical analogue of the HEL 87-97 sequence is obtained when 4 copies of this peptide sequence are coupled on a peptide carrier molecule following the template-assembled synthetic protein (TASP) approach [M. Mutter and S. Vuilleumier (1989) Angew. Chem. Int. Ed. Engl., Vol. 28, pp. 535-554 "A Chemical Approach to Protein Design-Template-Assembled Synthetic Proteins (TASP)." This suggests that long-range interactions of the peptide with its environment contribute to conformational stability in short peptide sequences. TASP molecules may prove useful for the study of the factors that determine secondary structure formation in short peptides by providing a protein-like framework.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 047517778

Download citation: RISBibTeXText

Related references

Analysis of peptide design in four-, five-, and six-helix bundle template assembled synthetic protein molecules. Biopolymers 88(5): 774-779, 2007

Synthesis of a 4 helix bundle like template assembled synthetic protein tasp by condensation of a protected peptide on a conformationally constrained cyclic carrier. Tetrahedron Letters 31(28): 4015-4018, 1990

Molecular dynamics conformational search of six cyclic peptides used in the template assembled synthetic protein approach for protein de novo design. Biopolymers 32(10): 1283-1310, 1992

The construction of new proteins: V. A template-assembled synthetic protein (TASP) containing both a 4-helix bundle and beta-barrel-like structure. Proteins 5(1): 13-21, 1989

Template assembled synthetic proteins Condensation of a multifunctional peptide to a topological template via chemoselective ligation. Tetrahedron Letters 34(52): 8419-8422, 1993

Template assembled synthetic proteins with four helix bundle topology total chemical synthesis and conformational studies. Journal of the American Chemical Society 114(4): 1463-1470, 1992

Crystal structure of a synthetic cyclodecapeptide for template-assembled synthetic protein design. Chembiochem: a European Journal of Chemical Biology 2(6): 432-437, 2001

Preparation of a verifiable peptide-protein immunogen: direction-controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis. Analytical Biochemistry 171(2): 277-282, 1988

Inhibition of the interaction between protein s and c4b binding protein by a synthetic peptide from the protein s sequence. Arteriosclerosis 8(5): 683A-684A, 1988

Antigen presentation of lysozyme: T-cell recognition of peptide and intact protein after priming with synthetic overlapping peptides comprising the entire protein chain. Immunology 56(1): 103-112, 1985

Conformational study of a short Pertussis toxin T cell epitope incorporated in a multiple antigen peptide template by CD and two-dimensional NMR. Analysis of the structural effects on the activity of synthetic immunogens. European Journal of Biochemistry 217(1): 171-187, 1993

A template-assembled model of the N-peptide helix bundle from HIV-1 Gp-41 with high affinity for C-peptide. Chemical Biology and Drug Design 70(4): 319-328, 2007

Conformational variability of the synthetic peptide 129-141 of the mouse prion protein. Journal of Biomolecular Structure and Dynamics 18(2): 237-248, 2000

Synthetic peptide arrays and peptide combinatorial libraries for the exploration of protein-ligand interactions and the design of protein inhibitors. Combinatorial Chemistry and High Throughput Screening 8(2): 135-143, 2005

Synthetic Fc peptide-mediated regulation of the immune response. I. Characterization of the immunomodulating properties of a synthetic 23-amino acid peptide derived from the sequence of the CH3 domain of human IgG1. Journal of Experimental Medicine 157(3): 947-956, 1983