The Cloning and Construction of a Murine-human Chimeric Antibody with Specificity for HBsAg and the Expression of the Chimeric Heavy Chain in E. coli
Chen, Y.-H.; Zhou, G.; Wu, X.-F.; Yu, Y.-J.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 28(3): 257-264
The genes encoding the heavy and light chain variable regions (V(H) and V(K)) of the murine monoclonal antibody with the specificity for hepatitis B virus surface antigen (HBsAg) have been clone by using RT-PCR. They were combined to the Human C(gamma)(3) and C(K) genes respectively to construct the murine human chimeric antibody genes The chimeric heavy chain was expressed in E. coli, and characterized by SDS-PAGE, Western-blot, indirect ELISA Assay to demonstrate the expression product has the HBsAg-binding ability.