The effect of inhibitors of DNA and protein synthesis on the chromosome aberration yield in a human lymphocyte culture exposed to gamma and neutron irradiation at different stages of the mitotic cycle. The cytogenetic effects in the G0 phase

Golub, E.V.; Bogatykh, B.A.; Sevan'kaev, A.V.

Radiatsionnaia Biologiia Radioecologiia 34(2): 183-189


ISSN/ISBN: 0869-8031
PMID: 8193704
Accession: 047621371

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The effect of inhibitors of protein synthesis, cycloheximide (CH), and DNA synthesis, hydroxyurea (OU) in combination with 5-fluoro-2-deoxyuridine (OU + FDUR), on the yield of chromosome aberrations in cultured human lymphocytes induced by gamma- and neutron irradiation with doses of 1 and 2 Gy in G0 phase of mitotic cycle has been studied. The inhibitors added immediately after gamma-irradiation have caused reliable increase of aberration rate (CH at both doses and OU + FDUR at a dose of 2 Gy only). Both inhibitors caused no effect being added after 1.5, 3 and 5 hr after gamma-irradiation. The chromosome aberration rate substantially increased at both doses of neutron irradiation for both inhibitors added at all studied time intervals after exposure. Chromosome exchange aberrations have most contributed to the increase of aberration rate.