+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The inhibition of ribonucleic acid synthesis by the thiol-oxidizing agent, diamide, in Escherichia coli



The inhibition of ribonucleic acid synthesis by the thiol-oxidizing agent, diamide, in Escherichia coli



Febs Letters 9(2): 100-102



The thiol-oxidizing agent, diamide, has been used to convert glutathione to glutathione disulfide within the cells of a stringent strain of Escherichia coli (CP 78), leading to a cessation of 14C-leucine incorporation (protein synthesis) and 3H-uracil incorporation (RNA synthesis). Parallel experiments with an isogenic relaxed strain (CP 79) gave similar results, providing evidence that glutathione is closely linked to RNA synthesis indepently of the link previously shown to protein synthesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 047668201

Download citation: RISBibTeXText

PMID: 11947641

DOI: 10.1016/0014-5793(70)80323-4


Related references

Effect of the thiol-oxidizing agent diamide on the growth of Escherichia coli. Journal of Bacteriology 101(3): 1092-1093, 1970

Continued expression of the ribonucleic acid control gene during inhibition of Escherichia coli ribonucleic acid and protein synthesis. Journal of Bacteriology 102(3): 702-710, 1970

Interaction of ribonucleic acid polymerase from Escherichia coli with deoxyribonucleic acid. Inhibition of ribonucleic acid synthesis by interaction of luteoskyrin with the transcription complex. Biochemistry 12(12): 2318-2324, 1973

Glutathione. VII. Differentiation among substrates by the thiol-oxidizing agent, diamide. Biochimica et Biophysica Acta 264(1): 39-44, 1972

Stimulation of septation in mitochondria by diamide a thiol oxidizing agent. Cell & Tissue Research 203(2): 291-300, 1979

The effect of diamide a thiol oxidizing agent on isolated adult rat myocytes. Journal of Pathology 131(3): 257, 1980

Effects of diamide a thiol oxidizing agent on frog skin permeability. Experientia 33(6): 780, 1977

Tryptophanyl transfer ribonucleic acid synthetase of Escherichia coli. Character of required thiol group and structure of thiol peptides. Journal of Biological Chemistry 251(11): 3254-3260, 1976

Role of glutathione in reversing the deleterious effects of a thiol-oxidizing agent in Escherichia coli. Journal of Bacteriology 133(3): 1150-1155, 1978

Effect of the thiol-oxidizing agent, diamide, on acetylcholine release at the frog endplate. Nature: New Biology 233(38): 120-121, 1971

Uncoupling of crayfish septate giant axons by diamide a thiol oxidizing agent. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles 289(14): 1025-1028, 1979

Transient growth inhibition of Escherichia coli K-12 by ion chelators: "in vivo" inhibition of ribonucleic acid synthesis. Journal of Bacteriology 138(3): 923-932, 1979

The synthesis of ribonucleic acid during inhibition of Escherichia coli by chlortetracycline. Biochemical Journal 97(1): 277-283, 1965

Glutathione. V. The effects of the thiol-oxidizing agent diamide on initiation and translation in rabbit reticulocytes. Biochimica et Biophysica Acta 228(1): 245-251, 1971

Effect of the thiol-oxidizing agent diamide on NH2Cl-induced rat colonic electrolyte secretion. American Journal of Physiology 265(1 Pt 1): C166-C170, 1993