+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The spike protein of transmissible gastroenteritis coronavirus controls the tropism of pseudorecombinant virions engineered using synthetic minigenomes



The spike protein of transmissible gastroenteritis coronavirus controls the tropism of pseudorecombinant virions engineered using synthetic minigenomes



Advances in Experimental Medicine and Biology 440: 207-214



The minimum sequence required for the replication and packaging of transmissible gastroenteritis virus (TGEV)-derived minigenomes has been determined. To this end, cDNAs encoding defective RNAs have been cloned and used to express heterologous spike proteins, to determine the influence of the peplomer protein in the control of TGEV tropism. A TGEV defective interfering RNA of 9.7 kb (DI-C) was isolated, and a cDNA complementary to DI-C RNA was cloned under the control of T7 promoter. In vitro transcribed DI-C RNA was replicated in trans upon transfection of helper virus-infected cells. A collection of DI-C deletion mutants (TGEV minigenomes) was generated and tested for their ability to be replicated and packaged. The size of the smallest minigenome replicated in trans was 3.3 kb. The rescue system was used to express the spike protein of an enteric TGEV isolate (C11) using as helper virus a TGEV strain (C8) that replicates very little in the gut. A mixture of two pseudorecombinant viruses containing either the helper virus genome or the minigenome was obtained. These pseudorecombinants display in the surface the S proteins from the enteric and the attenuated virus, and showed 10(4)-fold increase in their gut replication levels as compared to the helper isolate (C8). In addition, the pseudorecombinant virus increased its enteric pathogenicity as compared to the C8 isolate.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 047740058

Download citation: RISBibTeXText

PMID: 9782282


Related references

Replication and packaging of transmissible gastroenteritis coronavirus-derived synthetic minigenomes. Journal of Virology 73(2): 1535-1545, 1999

Two amino acid changes at the N-terminus of transmissible gastroenteritis coronavirus spike protein result in the loss of enteric tropism. Virology 227(2): 378-388, 1997

Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. Journal of Virology 73(9): 7607-7618, 1999

In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes. Journal of General Virology 83(Pt 3): 567-579, 2002

Expression of transcriptional units using transmissible gastroenteritis coronavirus derived minigenomes and full-length cDNA clones. Advances in Experimental Medicine and Biology 494: 447-451, 2001

The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets. Viruses 11(4):, 2019

Cloning and sequence analysis of spike protein gene of swine transmissible gastroenteritis coronavirus. Journal of Northwest A and F University Natural Science Edition 35(7): 1-6, 2007

Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus. Archives of Virology 145(8): 1725-1732, 2000

Evolution and tropism of transmissible gastroenteritis coronavirus. Advances in Experimental Medicine and Biology 342: 35-42, 1993

Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells. Virus Research 128(1-2): 52-57, 2007

Major receptor-binding and neutralization determinants are located within the same domain of the transmissible gastroenteritis virus (coronavirus) spike protein. Journal of Virology 68(12): 8008-8016, 1994

Complete genomic sequences, a key residue in the spike protein and deletions in nonstructural protein 3b of US strains of the virulent and attenuated coronaviruses, transmissible gastroenteritis virus and porcine respiratory coronavirus. Virology 358(2): 424-435, 2007

Infection of porcine precision cut intestinal slices by transmissible gastroenteritis coronavirus demonstrates the importance of the spike protein for enterotropism of different virus strains. Veterinary Microbiology 205: 1-5, 2017

Induction of an immune response to transmissible gastroenteritis coronavirus using vectors with enteric tropism. Advances in Experimental Medicine and Biology 342: 455-462, 1993

Induction of transmissible gastroenteritis coronavirus specific immune responses using vectors with enteric tropism. Advances in Experimental Medicine and Biology 371b: 1535-1541, 1995