+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The survival identification of pancreatic islets of Langerhans. In vitro and in vivo effects of two dithizone preparations on staining of rat and human islets of Langerhans-preliminary study (Part I)



The survival identification of pancreatic islets of Langerhans. In vitro and in vivo effects of two dithizone preparations on staining of rat and human islets of Langerhans-preliminary study (Part I)



Acta Poloniae Pharmaceutica 52(5): 431-436



Dithizone (DTZ) which selectively stains islets of Langerhans in vitro has a diagnostic potential in the in vivo identification of viable transplanted pancreatic islets. In the present study, we compared the use of DTZ, and a synthetic iodo-derivative of DTZ (I-DTZ) as a potential radioactive marker for identification of viable transplanted pancreatic islet cells. Fresh islets isolated from Lewis rat donors were transplanted beneath the kidney capsule into each diabetic syngeneic (Lewis) recipient rat. DTZ(I-DTZ) solution was injected intravenously (i.v.) at a dose 10 mg/kg body weight (b.w.) for macro and microscopic identification of surviving transplanted islets. The recipients were nephrectomized to confirm that the normoglycemic state was maintained by the islet grafts. In addition, we administered i.v. at the same time concentrations of DTZ (I-DTZ) to test for toxicity. The results show that following i.v. or intraductal (i.d.) injection of low concentrations does not damage pancreatic islet function as determined by insulin secretion or glucose levels. Angiogenesis and microvascularization were observed for 10-12 days after transplantation, and the histologic and biochemical studies showed no pathological changes in injected rats in organ examined. The human pancreatic islets harvested from multiorgan donors stain red ex vivo in the same way as DTZ or I-DTZ administered i.v. in rats. The results indicate that DTZ has a diagnostic potential in monitoring the survival of transplanted pancreatic islets and possibly in the early diagnosis by radioisotopic detection of pancreatic endocrine diseases.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 047744860

Download citation: RISBibTeXText

PMID: 8960276


Related references

Dietary factors and quantitative morphology of the islets of Langerhans. An experimental study of the influence of the caloric composition of the diet on the islets of Langerhans and an analysis of the relation between the volume of islet tissue and the number of islets in the rat. Acta med. scand, 128: Suppl. 198, 154,, 1947

A Historical Perspective on the Identification of Cell Types in Pancreatic Islets of Langerhans by Staining and Histochemical Techniques. Journal of Histochemistry and Cytochemistry 63(8): 543-558, 2015

Survival of cryopreserved isolated adult human pancreatic islets of Langerhans. Transplantation 44(1): 75-82, 1987

Expression of complement regulatory proteins on islets of Langerhans: a comparison between human islets and islets isolated from normal and hDAF transgenic pigs. Transplantation 72(2): 312-319, 2001

Expression Of Complement Regulatory Proteins On Islets Of Langerhans: A Comparison Between Human Islets And Islets Isolated From Normal And hDaf Transgenic Pigs1. Transplantation 72(2): 312-319, 2001

Labeling of human pancreatic islets of Langerhans with Endorem (R) for non-invasive MRI imaging in vivo. 2007

An in vitro assessment of human fetal pancreatic islets of Langerhans in culture. Transplantation Proceedings 24(3): 1030-1031, 1992

A Differential Staining Method For A- And B-Cells In The Pancreatic Islets Of Langerhans. Acta Histochemica et Cytochemica 10(1): 10-13, 1977

Viral Infection of Human Fetal Islets of Langerhans: Replication of Human Cytomegalovirus in Cultured Human Fetal Pancreatic Islets. American Journal of Clinical Pathology 90(1): 52-57, 1988

Viral infection of human fetal islets of langerhans replications of human cytomegalovirus in cultured human fetal pancreatic islets. American Journal of Clinical Pathology 90(1): 52-57, 1988

Supravital dithizone staining in the isolation of human and rat pancreatic islets. Diabetes Research 10(2): 53-57, 1989

Prostacyclin--protective effects on human islets of Langerhans in vitro. Transplantation Proceedings 22(4): 2048-2049, 1990

In vitro staining of islets of Langerhans for fluorescence-activated cell sorting. Transplantation 52(3): 450-452, 1991

Hyperproliferative state of liver acini and pancreatic islets after intraportal transplantation of a small mass of islets of Langerhans in streptozotocininduced diabetic rats. Experimental and Clinical Endocrinology and Diabetes 103(Suppl. 2): 112-117, 1995

Proceedings of the 2nd international islet symposium of the wenner gren center on the structure and metabolism of the pancreatic islets a centennial of paul langerhans discovery section on pancreatic islets and diabetes umea sweden february 17 19 1969. Falkmer, S , B Hellman And I -B Taljedal (Edited By) Wenner-Gren Center International Symposium Series, Vol 16 The Structure And Metabolism Of The Pancreatic Islets Xii + 568p Illus Pergamon Press Inc : Elmsford, N Y , U S A , Oxford, England 497-543, 1970