+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Typification of strains of Helicobacter pylori by the detection of the cagA gene and subtypes of the vacA gene

Typification of strains of Helicobacter pylori by the detection of the cagA gene and subtypes of the vacA gene

Enfermedades Infecciosas y Microbiologia Clinica 17(4): 171-175

Helicobacter pylori infection is probably the most common chronic bacterial infection in the world. The consequences of infection are pathologies like peptic ulcer, chronic gastritis, gastric cancer and gastric MALT lymphoma. The aim of this study was to detect the vacuolating cytotoxin gene (vacA) type, the cytotoxin-associated gen (cagA) of H. pylori isolates and study their association with the vacuolising activity. Gastric biopsy specimens were obtained from dyspeptic patients. Isolates were further genotypically typed by PCR. All strains studied were vacA+ and 55% were cagA+. All cytotoxic strains were cagA+, subtype vacA s1/m1 and corresponded to patients with peptic ulceration. The cagA- strains (11) corresponded to subtype s2/m2. We didn't demonstrate vacuolising activity on subtypes s1/m2 and s2/m2. A high genetic diversity exists among strains in our environment. The subset of bacteria, vacA s1/m1/cagA+, is associated with vacuolising activity in culture cells (tox+).

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 047860635

Download citation: RISBibTeXText

PMID: 10365510

Related references

Detection of cagA gene and typing of vacA gene in Helicobacter pylori strains isolated in the Czech Republic. Abstracts of the General Meeting of the American Society for Microbiology 103: D-071, 2003

Mosaicism in vacA, the vacuolating cytotoxin gene of Helicobacter pylori, and genetic linkage of vacA subtypes with cagA, the cytotoxin-associated gene. Gastroenterology 108(4 Suppl. ): A774, 1995

Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization. Journal of Clinical Microbiology 36(5): 1271-1276, 1998

Construction of a prokaryotic expression system of vacA gene and detection of vacA gene, VacA protein in Helicobacter pylori isolates and ant-VacA antibody in patients' sera. World Journal of Gastroenterology 10(7): 985-990, 2004

Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan. Journal of Clinical Microbiology 35(7): 1710-1714, 1997

Differences in the inflammatory response induced by short-term infections with cagA+/vacA+ or cagA-/vacA- Helicobacter pylori strains in mice. Gut 39(Suppl. 2): A77, 1996

Differences of vacA alleles and cagA gene positivity of Helicobacter pylori strains isolated from two different countries: Turkey and Germany. Mikrobiyoloji Bulteni 46(2): 332-334, 2012

Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene, CagA protein in Helicobacter pylori isolates and its antibody in sera of patients. World Journal of Gastroenterology 10(8): 1183-1190, 2004

Detection of vacA by PCR and correlation with cagA in Helicobacter pylori strains isolated from patients with different diseases. Gut 37(Suppl. 1): A67, 1995

Detection and typing of the virulence determinants cagA and vacA of Helicobacter pylori directly from biopsy DNA: are in vitro strains representative of in vivo strains?. European Journal of Gastroenterology and Hepatology 10(8): 683-687, 1998

Direct detection and analysis of vacA genotypes and cagA gene of Helicobacter pylori from gastric biopsies by a novel multiplex polymerase chain reaction assay. Diagnostic Microbiology and Infectious Disease 62(4): 366-373, 2008

Helicobacter pylori vacA genotypes and cagA gene in a series of 383 H. pylori-positive patients. Zeitschrift für Gastroenterologie 38(7): 559-564, 2000

Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms. Gastroenterology 135(1): 91-99, 2008

Association between gastroduodenal diseases and cagA, vacA gene expressions of Helicobacter pylori. Zhonghua Er Ke Za Zhi 41(1): 56-57, 2003

Distinct variants of Helicobacter pylori cagA are associated with vacA subtypes. Journal of Clinical Microbiology 37(7): 2306-2311, 1999