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Ultrasonic separations in analytical biotechnology

Ultrasonic separations in analytical biotechnology

Trends in Biotechnology 15(12): 506-511

Cells in megahertz-frequency noncavitating ultrasonic standing waves concentrate at submillimetre distances and are, as large clumps, easily removed from suspension in flow or batch systems. An ultrasonic filter for perfusion hybridoma culture is now available. Results from small-volume prototype analytical-scale systems can inform the design of effective filter or batch-clarification systems for a wide range of cell sizes, concentrations and sample volumes. Large increases in the rates of aqueous biphasic separations and of the rates and sensitivities of analytical immunocoated particle-agglutination assays occur in standing waves. Ultrasonic manipulation is briefly compared with immunomagnetic and dielectrophoretic separations.

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Accession: 047865890

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PMID: 9418305

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