+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Use of 13C nuclear magnetic resonance to assess fossil fuel biodegradation: fate of [1-13C]acenaphthene in creosote polycyclic aromatic compound mixtures degraded by bacteria

Use of 13C nuclear magnetic resonance to assess fossil fuel biodegradation: fate of [1-13C]acenaphthene in creosote polycyclic aromatic compound mixtures degraded by bacteria

Applied and Environmental Microbiology 64(4): 1447-1453

[1-13C]acenaphthene, a tracer compound with a nuclear magnetic resonance (NMR)-active nucleus at the C-1 position, has been employed in conjunction with a standard broad-band-decoupled 13C-NMR spectroscopy technique to study the biodegradation of acenaphthene by various bacterial cultures degrading aromatic hydrocarbons of creosote. Site-specific labeling at the benzylic position of acenaphthene allows 13C-NMR detection of chemical changes due to initial oxidations catalyzed by bacterial enzymes of aromatic hydrocarbon catabolism. Biodegradation of [1-13C]acenaphthene in the presence of naphthalene or creosote polycyclic aromatic compounds (PACs) was examined with an undefined mixed bacterial culture (established by enrichment on creosote PACs) and with isolates of individual naphthalene- and phenanthrene-degrading strains from this culture. From 13C-NMR spectra of extractable materials obtained in time course biodegradation experiments under optimized conditions, a number of signals were assigned to accumulated products such as 1-acenaphthenol, 1-acenaphthenone, acenaphthene-1,2-diol and naphthalene 1,8-dicarboxylic acid, formed by benzylic oxidation of acenaphthene and subsequent reactions. Limited degradation of acenaphthene could be attributed to its oxidation by naphthalene 1,2-dioxygenase or related dioxygenases, indicative of certain limitations of the undefined mixed culture with respect to acenaphthene catabolism. Coinoculation of the mixed culture with cells of acenaphthene-grown strain Pseudomonas sp. strain A2279 mitigated the accumulation of partial transformation products and resulted in more complete degradation of acenaphthene. This study demonstrates the value of the stable isotope labeling approach and its ability to reveal incomplete mineralization even when as little as 2 to 3% of the substrate is incompletely oxidized, yielding products of partial transformation. The approach outlined may prove useful in assessing bioremediation performance.

(PDF emailed within 1 workday: $29.90)

Accession: 047891173

Download citation: RISBibTeXText

PMID: 9546181

Related references

Carbon-13 NMR studies of biodegradation of fossil fuel wastes Fate of 13C-1-acenaphthene in creosote PAH mixtures degraded by bacteria. Abstracts of the General Meeting of the American Society for Microbiology 94(0): 420, 1994

Use of 13C NMR to assess the biodegradation of 1-13C-labeled acenaphthene in the presence of creosote polynuclear hydrocarbons and naphthalene by mixed bacterial cultures. Abstracts of Papers American Chemical Society 211(1-2): ENVR 37, 1996

Statistical Impact of the Extent of Desorption, Compound Aging, and Bacteria Inoculation on Polycyclic Aromatic Hydrocarbon Biodegradation. Polycyclic Aromatic Compounds 22(2): 197-214, 2002

Biodegradation of photoirradiated polycyclic aromatic hydrocarbon constituents of creosote oil. Environmental Technology 21(8): 901-907, 2000

Simultaneous biodegradation of creosote-polycyclic aromatic hydrocarbons by a pyrene-degrading Mycobacterium. Applied Microbiology and Biotechnology 78(1): 165-172, 2007

Integrating biodegradation and electroosmosis for the enhanced removal of polycyclic aromatic hydrocarbons from creosote-polluted soils. Journal of Environmental Quality 36(5): 1444-1451, 2007

Hierarchy of Carbon Source Utilization in Soil Bacteria: Hegemonic Preference for Benzoate in Complex Aromatic Compound Mixtures Degraded by Cupriavidus pinatubonensis Strain JMP134. Applied and Environmental Microbiology 81(12): 3914-3924, 2016

Combination of biochar amendment and mycoremediation for polycyclic aromatic hydrocarbons immobilization and biodegradation in creosote-contaminated soil. Journal of Hazardous Materials 285: 259-266, 2015

Selective determination of acenaphthene in mixtures of three-ring polycyclic aromatic hydrocarbons by fluorescence quenching in micellar medium of cetylpyridinium bromide. Journal of Fluorescence 7(2): 147-153, 1997

Bioavailability assessment and environmental fate of polycyclic aromatic hydrocarbons in biostimulated creosote-contaminated soil. Chemosphere 63(10): 1648-1659, 2005

Adding sodium dodecyl sulfate and Pseudomonas aeruginosa UG2 biosurfactants inhibits polycyclic aromatic hydrocarbon biodegradation in a weathered creosote-contaminated soil. Applied microbiology and biotechnology 46(5-6): 638-646, 1996

Prospects for carbon-13 nuclear magnetic resonance analysis of solid fossil-fuel materials. Fuel 56(4): 354-358, 1977

Relations between the averaged 13C nuclear magnetic resonance chemical shift and the carcinogenic activity of polycyclic aromatic hydrocarbons. Chemical and Pharmaceutical Bulletin 40(8): 1981-1985, 1992

Polycyclic aromatic hydrocarbons in Australian coals; III, Structural elucidation by proton nuclear magnetic resonance spectroscopy. Organic Geochemistry 12(3): 261-271, 1988

Kinetics of biodegradation of mixtures of polycyclic aromatic hydrocarbons. Applied Microbiology and Biotechnology 60(3): 361-366, 2002