Section 48
Chapter 47,995

Beta-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis

Montijn, R.C.; Van Wolven, P.; De Hoog, S.; Klis, F.M.

Microbiology 143: 1673-1680


ISSN/ISBN: 1350-0872
PMID: 9168616
DOI: 10.1099/00221287-143-5-1673
Accession: 047994446

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Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-beta-glucan antiserum, indicating that they are linked to 1,6-beta-glucan. It is proposed that 1,6-beta-glucosylated cell wall proteins generally occur among ascomycetes.

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