+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gamma-Aminobutyric acid type A receptors in the rat brain can contain both gamma 2 and gamma 3 subunits, but gamma 1 does not exist in combination with another gamma subunit



Gamma-Aminobutyric acid type A receptors in the rat brain can contain both gamma 2 and gamma 3 subunits, but gamma 1 does not exist in combination with another gamma subunit



Molecular Pharmacology 45(6): 1061-1070



Antibodies specific for the gamma 1, gamma 2, and gamma 3 subunits of the gamma-aminobutyric acid (GABA)A receptor have been used to probe the composition of naturally occurring GABAA receptors in the rat brain. Most GABAA receptors contain at least one of these three subunits. The percentage of each, determined by immunoprecipitation of [3H]muscimol binding, was 11 +/- 1%, 59 +/- 3%, and 14 +/- 2% for gamma 1, gamma 2, and gamma 3 subunits, respectively. Receptors containing gamma 2 or gamma 3 subunits were labeled by benzodiazepine site ligands with high affinity, whereas gamma 1-containing receptors could be labeled only by [3H]muscimol. Receptors immunoprecipitated by anti-gamma 2 or anti-gamma 3 antibodies were labeled with [3H]Ro 15-1788 with similar affinities (Kd for anti-gamma 2-immunoprecipitated receptors, 1.9 nM; Kd for anti-gamma 3-immunoprecipitated receptors, 1.7 nM). Immunoprecipitation or Western blot analysis of GABAA receptors solubilized from rat cerebellar or whole-brain preparations indicated that gamma 1 was not present coassembled with any other gamma subunit. Western blot analysis of receptors purified on alpha-specific immunoaffinity resins showed that gamma 1 was predominantly assembled with the alpha 2 subunit. Some GABAA receptors may contain more than one type of gamma subunit. Quantitative immunoprecipitation and Western blot analysis both indicated that gamma 2 and gamma 3 subunits can exist in the same receptor complex. A large proportion of GABAA receptors immunopurified on a gamma 3 affinity resin also appeared to contain a gamma 2 subunit. In contrast, when receptors were purified on a gamma 2 affinity resin a small proportion also appeared to contain a gamma 3 subunit. We conclude that most gamma 1-containing receptors have no other gamma subunit in the same receptor complex but some GABAA receptors contain both gamma 2 and gamma 3 subunits.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 047996338

Download citation: RISBibTeXText

PMID: 8022401


Related references

Immunoaffinity purification of gamma-aminobutyric acidA (GABAA) receptors containing gamma 1-subunits. Evidence for the presence of a single type of gamma-subunit in GABAA receptors. Journal of Biological Chemistry 269(41): 25777-25782, 1994

Potentiation of gamma-aminobutyric acid-induced chloride currents by various benzodiazepine site agonists with the alpha 1 gamma 2, beta 2 gamma 2 and alpha 1 beta 2 gamma 2 subtypes of cloned gamma-aminobutyric acid type A receptors. Molecular Pharmacology 44(4): 866-870, 1993

Decreased benzodiazepine binding with little effect on gamma-aminobutyric acid binding in rat brain after treatment with antisense oligodeoxynucleotide to the gamma-aminobutyric acidA receptor gamma-2 subunit. Journal of Pharmacology and Experimental Therapeutics 287(2): 752-759, 1998

Characterization of bicuculline/baclofen-insensitive (rho-like) gamma-aminobutyric acid receptors expressed in Xenopus oocytes. II. Pharmacology of gamma-aminobutyric acidA and gamma-aminobutyric acidB receptor agonists and antagonists. Molecular Pharmacology 43(4): 609-625, 1993

The gamma-subunit governs the susceptibility of recombinant gamma-aminobutyric acid type A receptors to block by the nonimmobilizer 1,2-dichlorohexafluorocyclobutane (F6, 2N). Anesthesia and Analgesia 101(2): 401-6, Table of Contents, 2005

Benzodiazepine-insensitive mice generated by targeted disruption of the gamma 2 subunit gene of gamma-aminobutyric acid type A receptors. Proceedings of the National Academy of Sciences of the United States of America 92(17): 7749-7753, 1995

Human chromosomal localization of genes encoding the gamma 1 and gamma 2 subunits of the gamma-aminobutyric acid receptor indicates that members of this gene family are often clustered in the genome. Proceedings of the National Academy of Sciences of the United States of America 89(13): 5857-5861, 1992

Neurochemical studies on gamma aminobutyric acid receptor xii. solubilization of gamma aminobutyric acid a and gamma aminobutyric acid b receptors from synaptic membrane of bovine cerebral cortex. Japanese Journal of Pharmacology 39(Suppl.): 176P, 1985

Regional variations in the in vivo effects of gamma acetylenic gamma aminobutyric acid on gamma aminobutyric acid transaminase and glutamate decarboxylase activities in rat central nervous system retina vs. brain. Society for Neuroscience Abstracts 11(1): 284, 1985

Effect of gamma-vinyl gamma-aminobutyric acid on the gamma-aminobutyric acid receptor-coupled chloride ion channel in vesicles from the brain of the rat. Neuropharmacology 30(5): 423-427, 1991

Gamma aminobutyric acid b receptors but not gamma aminobutyric acid a receptors are involved in the effects of systemically administered gamma aminobutyric acid ergic drugs on locomotion in the rat. Boletin De Estudios Medicos Y Biologicos Universidad Nacional Autonoma De Mexico8: 113,, 1985

Reduced expression of gamma-aminobutyric acid type A/benzodiazepine receptor gamma 2 and alpha 5 subunit mRNAs in brain regions of flurazepam-treated rats. Molecular Pharmacology 45(4): 657-663, 1994

Gamma aminobutyric acid regulation of circadian responses to light in mammals involvement of gamma aminobutyric acid a and gamma aminobutyric acid b receptors. Society for Neuroscience Abstracts 12(1): 209, 1986

Relationship between epilepsy and gamma aminobutyric acid mediated membrane functions gamma aminobutyric acid carriers and gamma aminobutyric acid receptors. Acta Neurologica Scandinavica 70(3): 217-218, 1984

Identification of the camp dependent protein kinase and protein kinase c phosphorylation sites within the major intracellular domains of the beta 1 gamma 2s and gamma 2l subunits of the gamma aminobutyric acid type a receptor. Journal of Biological Chemistry 267(20): 14470-14476, 1992