A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis
Wang, T.; Magnarelli, L.A.; Anderson, J.F.; Gould, L.H.; Bushmich, S.L.; Wong, S.J.; Fikrig, E.
Vector Borne and Zoonotic Diseases 2(2): 105-109
2002
ISSN/ISBN: 1530-3667
PMID: 12653304
DOI: 10.1089/153036602321131904
Accession: 048110373
Recombinant West Nile virus envelope (E) protein was examined in enzyme-linked immunosorbent assay (ELISA) to detect antibodies elicited during West Nile virus infection. Horses (nine of 10) and humans (six of six) with confirmed West Nile virus infection had IgG and/or IgM antibodies to the E protein. Antibodies to the recombinant West Nile virus membrane and nonstructural 1 proteins were not detected in any of these sera. An E protein-based ELISA may aid in the serological diagnosis of West Nile virus infection.
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