+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antibacterial activity and mode of action of totarol against Staphylococcus aureus in carrot juice



Antibacterial activity and mode of action of totarol against Staphylococcus aureus in carrot juice



Journal of Food Science and Technology 55(3): 924-934



Food contaminated with pathogenic bacteria such as Staphylococcus aureus (S. aureus), represents a serious health risk to human beings. Totarol is an antibacterial novel phenolic diterpenes. In present study, the antibacterial activity of totarol against S. aureus was investigated in a food system. The antibacterial activity of totarol was determined by measuring the zones of inhibition and minimum inhibitory concentrations (MICs). The MICs for S. aureus strains were in the range of 2-4 μg/ml. The probable antibacterial mechanism of totarol was the alteration in cell membranes integrity and permeability, which leading to the leakage of cellular materials. The electric conductivity showed a time- and dose-dependent increasing manner, and we utilized totarol to induce the production of cytoplasmic β-galactosidase in S. aureus. Scanning electron microscopy and transmission electron microscopy analysis further confirmed that S. aureus cell membranes were damaged by totarol. The time-kill assay and detection of the kinetics of S. aureus deactivation in situ indicated that totarol has good preservative activities in a food model. Totarol successfully inhibited S. aureus development in carrot juice, at room temperature (25 °C) and in refrigerator (4 °C) respectively. Our works provided not only additional evidences in support of totarol being regarded as a natural antibacterial food preservative but also fundamental understanding on the mode of antibacterial action. It is necessary to consider that totarol will become a promising antibacterial additive for food preservative.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048149077

Download citation: RISBibTeXText

PMID: 29487434

DOI: 10.1007/s13197-017-3000-2


Related references

Antibacterial mode of action of violacein from Chromobacterium violaceum UTM5 against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Environmental Science and Pollution Research International 25(6): 5164-5180, 2018

Effects of two terpene alcohols on the antibacterial activity and the mode of action of farnesol against Staphylococcus aureus. Molecules 13(12): 3069-3076, 2008

Antibacterial activity and mode of action of plant flavonoids against proteus vulgaris and staphylococcus aureus. Phytochemistry 26(8): 2231-2234, 1987

Studies on the mode of action of the phenolic antibacterial agent fentichlor against Staphylococcus aureus and Escherichia coli. I. The adsorption of fentichlor by the bacterial cell and its antibacterial activity. Journal of Applied Bacteriology 34(3): 557-567, 1971

Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin-resistant Staphylococcus aureus. Journal Of Applied Bacteriology. 80(4): 387-394, 1996

Studies on the antibacterial activity of phanquone: chelating properties in relation to mode of action against Escherichia coli and Staphylococcus aureus. Microbios 29(116): 109-125, 1980

Synergistic Antibacterial Effect and Antibacterial Action Mode of Chitosan-Ferulic Acid Conjugate against Methicillin-Resistant Staphylococcus aureus. Journal of Microbiology and Biotechnology 26(4): 784-789, 2016

Mode of antibacterial action of totarol, a diterpene from Podocarpus nagi. Planta Medica 62(2): 122-125, 1996

The antibacterial effects of terpene alcohols on Staphylococcus aureus and their mode of action. FEMS Microbiology Letters 237(2): 325-331, 2004

Retapamulin: an antibacterial with a novel mode of action in an age of emerging resistance to Staphylococcus aureus. Journal of Drugs in Dermatology 9(10): 1198-1204, 2010

Antibacterial mode of action of 1,8-dihydroxy-anthraquinone from Porphyra haitanensis against Staphylococcus aureus. Natural Product Research 29(10): 976-979, 2015

Elucidation of the Mode of Action of a New Antibacterial Compound Active against Staphylococcus aureus and Pseudomonas aeruginosa. Plos one 11(5): E0155139, 2016

Studies on the mode of action of the phenolic antibacterial agent fentichlor against Staphylococcus aureus and Escherichia coli. 3. The effect of fentichlor on the metabolic activities of Staphylococcus aureus and Escherichia coli. Journal of Applied Bacteriology 34(3): 579-591, 1971

Antibacterial Effects and Mode of Action of Selected Essential Oils Components against Escherichia coli and Staphylococcus aureus. Evidence-BasedComplementaryandAlternativeMedicine2015:795435, 2015

The phenolic diterpene totarol inhibits multidrug efflux pump activity in Staphylococcus aureus. Antimicrobial Agents and ChemoTherapy 51(12): 4480-4483, 2007