+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Activities of Ribulose Bisphosphate Carboxylase and Phosphoenolpyruvate Carboxylase and C-Bicarbonate Fixation during in Vitro Culture of Pinus radiata Cotyledons



Activities of Ribulose Bisphosphate Carboxylase and Phosphoenolpyruvate Carboxylase and C-Bicarbonate Fixation during in Vitro Culture of Pinus radiata Cotyledons



Plant Physiology 87(3): 675-679



The activities of ribulose bisphosphate carboxylase (RuBPC) and phosphoenolpyruvate carboxylase (PEPC), as indicators of autotrophic and nonautotrophic CO(2) fixation, were measured in excised cotyledons of Pinus radiata D. Don cultured for 21 days under shoot-forming (SF) and nonshoot-forming (NSF) conditions. The activity of RuBPC was found to increase in both SF and NSF cultures during the initial 5 days of culture. However, it leveled off from day 5 to day 10 and subsequently began to decrease until the end of the culture period under the SF conditions. In contrast, in the NSF cultures, RuBPC activity increased until day 15, when it was twofold higher than the maximum activity found in the SF cultures. An increase in PEPC activity of about 2.5 times the level of activity in freshly excised cotyledons was observed during the initial 5 days of culture under the SF conditions. PEPC activity began to decline after day 5 until it reached the level of activity seen in NSF cotyledons by day 15. In contrast, the activity of PEPC did not show any significant increase during the initial 10 days of culture under the NSF conditions. The K(m) (phosphoenolpyruvate) of PEPC from SF cotyledons was about 35% higher than that of NSF cotyledons. Cotyledons from two culture periods (days 5 and 15) were incubated for 15 seconds with NaH(14)CO(3). The label in the malate and asparatate fractions as a percentage of total (14)C incorporation was 3 times higher in the SF cotyledons than in the NSF cotyledons. A higher incorporation of (14)C into products of photosynthesis under the NSF conditions was also observed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048165554

Download citation: RISBibTeXText

PMID: 16666206

DOI: 10.1104/pp.87.3.675


Related references

Activities of ribulose bisphosphate carboxylase and phosphoenolpyruvate carboxylase and carbon 14 bicarbonate fixation during in vitro culture of pinus radiata cotyledons. Plant Physiology 87(3): 675-679, 1988

Changes in ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activities and 14CO2 fixation during the rooting of strawberry shoots in vitro. Canadian Journal of Plant Science 74(4): 827-831, 1994

Carbon fixation in Pinus halepensis submitted to ozone. Opposite response of ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase. Physiologia Plantarum 105(2): 187-192, 1999

Ribulose 1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase activities during micropropagation of Juglans regia plantlets. Photosynthetica 31(1): 141-146, 1995

Activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase, and oxygen evolution in transgenic tobacco plants. Biologia Plantarum 44(3): 447-450, 2001

The altered activities of carbonic anhydrase, phosphoenolpyruvate carboxylase and ribulose bisphosphate carboxylase due to water stress and after its relief. Journal of Environmental Biology 17(1): 39-42, 1996

Activities of chlorophyllase phosphoenolpyruvate carboxylase and ribulose 1 5 bisphosphate carboxylase in the primary leaves of soybean during senescence and drought. Physiologia Plantarum 81(4): 473-480, 1991

Dynamics of ribulose-1,5-bisphosphate carboxylase- and phosphoenolpyruvate carboxylase activities in a natural phytoplankton succession in relationship to primary production. Limnologica 22(4): 329-335, 1992

Dynamics of ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase activities in a natural phytoplankton succession in relation to primary production. ICES Marine Science Symposia 197: 284, 1993

Effect of Ear Removal on CO(2) Exchange and Activities of Ribulose Bisphosphate Carboxylase/Oxygenase and Phosphoenolpyruvate Carboxylase of Maize Hybrids and Inbred Lines. Plant Physiology 84(2): 261-265, 1987

Activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase/oxygenase in leaves and fruit pericarp tissue of different coffee (Coffea sp.) genotypes. Photosynthetica 38(2): 215-220, 2000

Ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase activities of photoautotrophic callus of Platycerium coronarium (Koenig ex O.F. Muell.) Desv. under CO2 enrichment. Plant Cell, Tissue and Organ Culture 50(2): 75-82, 1997

Activities of phosphoenolpyruvate carboxylase and ribulose 1 5 bisphosphate carboxylase from leaves of maize grown under different irrigation nitrate fertilization and stand density. Photosynthetica 23(3): 395-398, 1989

Carbon dioxide exchange rates, ribulose bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activities, and kernel growth characteristics of maize. Plant Physiology 84(2): 255-260, 1987

Temperature response of photosynthesis and activities of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase in greened calli induced iceplant, Mesembryanthemum crystallinum L, leaves. Japanese Journal Of Crop Science. 66(4): 531-537,., 1997