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Amplification and cloning of the N gene of SARS-associated coronavirus



Amplification and cloning of the N gene of SARS-associated coronavirus



Di 1 Jun Yi Da Xue Xue Bao 24(1): 39-41



To amplify and clone the N gene of severe acute respiratory syndrome-associated coronavirus. Using primer Premier 5.0 software, two pairs of nested PCR primers were designed to amplify the N gene. After purification, the amplified products were cloned into pMD18-T vectors, and the positive clones with the inserted fragments were identified by sequence analysis. The amplified products was about 1 375 bp in length, and sequence analysis demonstrated that the N gene fragments had been successfully inserted into pMD18-T vectors. The successful amplification and cloning of N gene facilitates further investigation of the expression of the N protein and study of its structure and functions.

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Accession: 048220330

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PMID: 14724092


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