+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Analyzing the H19- and T65-epitopes in 38 kd phosphorylated protein of Marek's disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes

Analyzing the H19- and T65-epitopes in 38 kd phosphorylated protein of Marek's disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes

Science in China. Series C, Life Sciences 47(1): 82-91

DNA sequencing analysis in 38 kd phosphorylated protein (pp38) ORF of Marek's disease viruses (MDV) indicated that all tested 10 virulent strains with different pathotypes had 'A' at base #320 and glutamine at aa#107 while reacted with monoclonal antibody (Mab) H19 in indirect fluorescence antibody test (IFA). However, vaccine strain CVI988 had 'G' at base#320 and arginine at aa#107 instead, when it was negative in IFA with Mab H19. Some strains were also reactive with Mab T65 in IFA while there was 'G' at base #326 and glycine at aa#109, but the other strains, which had 'A' at base #326 and glutamic acid at aa#109, did not react with Mab T65. By comparison of CVI988 to its point mutants CVI/rpp38(AG) and CVI/rpp38(AA) with 1 or 2 base(s) changes at bases #320 and /or #326 of pp38 gene for their reactivity with Mab H19 and T65, it was confirmed that the glutamine at aa#107 and glycine at aa#109 were critical to epitopes H19 and T65 respectively. Immuno-reactions to MDV were compared in SPF chickens inoculated with cloned CVI988 and its mutant CVI/rpp38(AG). It was found that antibody responses to MDV in chickens inoculated with CVI/rpp38(AG) were delayed and significantly lower than that in chickens inoculated with the native CVI988. By differential comparison of antibody titers to different antigens, a third epitope specific to CVI988 and dependent on arginine at aa#107 was suggested to be responsible for the big difference in antibody responses induced by native CVI988 and its mutant.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 048254077

Download citation: RISBibTeXText

PMID: 15382680

DOI: 10.1360/02yc0173

Related references

Immunological cross-reaction of serotype I Marek's disease virus 38kD phosphorylated protein with serotype II and III viruses. Wei Sheng Wu Xue Bao 34(5): 393-397, 1994

Construction and characterization of point-mutated Marek's disease virus vaccine strain CVI988 expressing the virulent epitope on its 38 kDa phosphorylated protein. Chinese Journal of Virology 15(2): 147-153, 1999

The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virus. Journal of General Virology 83(Pt 9): 2201-2209, 2002

Monoclonal antibodies against different epitopes of a 40 Kd capsid protein of infectious bursal disease viruses. Proceedings of the National Science Council, Republic of China. Part B, Life Sciences 14(2): 75-84, 1990

Studies on Marek's disease: propagation and cytopathic changes by local strains of Marek's disease viruses in chicken kidney cell-culture. Indian Journal of Animal Sciences 1977; 45(12): 962-966, 1976

Efficient generation and rapid isolation via stoplight recombination of Herpes simplex viruses expressing model antigenic and immunological epitopes. Journal of Virological Methods 179(1): 116-126, 2012

Comparing DNA sequences in glycoprotein I gene of Marek's disease viruses of different pathotypes. Virologica Sinica 15(2): 180-187, 2000

Interaction between Marek's disease and chicken infectious anaemia viruses. Selezione Veterinaria ( 10): 727-742, 1999

Common epitopes shared between streptococcal m protein and viruses may be a link to autoimmunity. Orefici, G (Ed ) Zentralblatt Fuer Bakteriologie Supplement, 22 New Perspectives on Streptococci And Streptococcal Infections; (International Journal Of Medical Microbiology, 22 New Perspectives on Streptococci And Streptococcal Infections); Xi Lancefield International Symposium on Streptococci And Streptococcal Diseases, Siena, Italy, September 10-14, 1990 Xix+569p Gustav Fischer Verlag: Stuttgart, Germany; New York, New York, Usa Illus 534-536, 1992

Common epitopes shared between streptococcal m protein and viruses are a link to autoimmunity. FASEB Journal 4(7): A2228, 1990

Influenza A viruses expressing epitopes of protein F of Pseudomonas aeruginosa within their hemagglutinins. Abstracts of the General Meeting of the American Society for Microbiology 95(0): 582, 1995

Comparison of neutralizing epitopes among infectious bursal disease viruses using radioimmunoprecipitation. Avian Diseases 39(3): 499-506, 1995

Effect of diluting Marek's disease vaccines on the outcomes of Marek's disease virus infection when challenged with highly virulent Marek's disease viruses. Avian Diseases 55(2): 263-272, 2011

Attenuation of recombinant yellow fever 17D viruses expressing foreign protein epitopes at the surface. Journal of Virology 79(13): 8602-8613, 2005

Mapping of two immunodominant antigenic epitopes conserved among the major inner capsid protein, VP7 of five bluetongue viruses. Virology 178(2): 552-559, 1990