+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Characterization of the CaENG1 gene encoding an endo-1,3-beta-glucanase involved in cell separation in Candida albicans

Characterization of the CaENG1 gene encoding an endo-1,3-beta-glucanase involved in cell separation in Candida albicans

Current Microbiology 51(6): 385-392

ISSN/ISBN: 0343-8651

PMID: 16328626

DOI: 10.1007/s00284-005-0066-2

The Candida albicans CaENG1 gene encoding an endo-1,3-beta-glucanase was cloned by screening a genomic library with a DNA probe obtained by polymerase chain reaction using synthetic oligonucleotides designed according to conserved regions found between two Saccharomyces cerevisiae endo-1,3-beta-glucanases (Eng1p and Eng2p). The gene contains a 3435-bp open reading frame (ORF), capable of encoding a protein of 1145 amino acids (124,157 Da), that contains no introns. Comparison of the ScEng1p sequence with partial C. albicans genomic sequences revealed the presence of a second protein with sequence similarity (the product of the Ca20C1.22c ORF, which was named CaENG2). Disruption of the CaENG1 gene in C. albicans had no dramatic effects on the growth rate of the strains, but it resulted in the formation of chains of cells, suggesting that the protein is involved in cell separation. Expression of CaENG1 in S. cerevisiae cells afforded a 12-fold increase in the 1,3-beta-glucanase activity detected in culture supernatants, showing that the protein has similar enzymatic activity to that of the S. cerevisiae Eng1p. In addition, when the C. albicans protein was expressed under its native promoter in S. cerevisiae eng1 mutant cells, it was able to complement the separation defect of this mutant, indicating that these two proteins are true functional homologues.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 048498198

Download citation: RISBibTeXText

Related references

Over-expression of the Saccharomyces cerevisiae exo-beta-1,3-glucanase gene together with the Bacillus subtilis endo-beta-1,3-1,4-glucanase gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in yeast. Journal of Biotechnology 55(1): 43-53, 1997

Cloning and characterization of the gene encoding endo-beta-1,3-glucanase from Arthrobacter sp. NHB-10. Bioscience Biotechnology and Biochemistry 71(6): 1568-1571, 2007

Expression and characterization of the celcflB gene from Cellulomonas flavigena encoding an endo-beta-1,4-glucanase. Current Microbiology 47(5): 359-363, 2003

Isolation and characterization of a gene encoding endo-beta-1,4-glucanase from pepper (Capsicum annuum L.). Plant Molecular Biology 33(1): 47-59, 1997

Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis. Applied Microbiology and Biotechnology 38(4): 507-513, 1993

Multiple endo-beta-1,4-glucanase-encoding genes from Bacillus lautus PL236 and characterization of the celB gene. Gene 93(1): 55-60, 1990

Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae. Eukaryotic Cell 1(5): 774-786, 2002

Characterization of caEG2, a pepper endo- beta -1,4-glucanase gene involved in the abscission of leaves and flowers. Biology and biotechnology of the plant hormone ethylene II Proceedings of the EU TMR Euroconference Symposium, Thira Santorini, Greece, 5-8 September 1998: 269-270, 1999

Safety and efficacy of FRA ® Octazyme C Dry (endo-1,4-β-xylanase, mannan-endo-1,4-β-mannosidase, α-amylase, endo-1,3(4)-β-glucanase, pectinase, endo-1,4-β-glucanase, protease, α-galactosidase) as a feed additive for weaned piglets and chickens for fattening. Efsa Journal. European Food Safety Authority 17(6): E05730, 2019

A gene encoding endo-1,4-beta-glucanase from Bacillus sp. 22-28. Bioscience Biotechnology and Biochemistry 61(2): 362-364, 1997

Cloning and characterization of a cDNA encoding the endo-1,4-beta-D-glucanase gene expressed in rapidly growing tuber and leaf of taro (Colocasia esculenta var. esculenta). Botanical Studies 50(4): 379-387, 2009

Cloning and characterization of a gene encoding a cell surface protein of Candida albicans. Abstracts of the General Meeting of the American Society for Microbiology 97: 596, 1997

Over-expression of the gene (bglBC1) from Bacillus circulans encoding an endo-beta-(1-->3),(1-->4)-glucanase useful for the preparation of oligosaccharides from barley beta-glucan. Biotechnology Letters 26(22): 1749-1755, 2004

Purification and characterization of the Saccharomyces cerevisiae BGL2 gene product, a cell wall endo-beta-1,3-glucanase. Journal of Bacteriology 175(7): 2102-2106, 1993

Separation and characterization of the endo beta 1 3 d glucanase from rhizoctonia solani. Agricultural and Biological Chemistry 50(3): 543-550, 1986