+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chronic post-ischemia pain (CPIP): a novel animal model of complex regional pain syndrome-type I (CRPS-I; reflex sympathetic dystrophy) produced by prolonged hindpaw ischemia and reperfusion in the rat



Chronic post-ischemia pain (CPIP): a novel animal model of complex regional pain syndrome-type I (CRPS-I; reflex sympathetic dystrophy) produced by prolonged hindpaw ischemia and reperfusion in the rat



Pain 112(1-2): 94-105



A neuropathic-like pain syndrome was produced in rats following prolonged hindpaw ischemia and reperfusion, creating an animal model of complex regional pain syndrome-Type I (CRPS-I; reflex sympathetic dystrophy) that we call chronic post-ischemia pain (CPIP). The method involves placing a tourniquet (a tight fitting O-ring) on one hindlimb of an anesthetized rat just proximal to the ankle joint for 3 h, and removing it to allow reperfusion prior to termination of the anesthesia. Rats exhibit hyperemia and edema/plasma extravasation of the ischemic hindpaw for a period of 2-4 h after reperfusion. Hyperalgesia to noxious mechanical stimulation (pin prick) and cold (acetone exposure), as well as mechanical allodynia to innocuous mechanical stimulation (von Frey hairs), are evident in the affected hindpaw as early as 8 h after reperfusion, and extend for at least 4 weeks in approximately 70% of the rats. The rats also exhibit spontaneous pain behaviors (hindpaw shaking, licking and favoring), and spread of hyperalgesia/allodynia to the uninjured contralateral hindpaw. Light-microscopic examination of the tibial nerve taken from the region just proximal to the tourniquet reveals no signs of nerve damage. Consistent with the hypothesis that the generation of free radicals may be partly responsible for CRPS-I and CPIP, two free radical scavengers, N-acetyl-L-cysteine (NAC) and 4-hydroxy-2,2,6,6-tetramethylpiperydine-1-oxyl (Tempol), were able to reduce signs of mechanical allodynia in this model.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048522164

Download citation: RISBibTeXText

PMID: 15494189

DOI: 10.1016/j.pain.2004.08.001


Related references

The Therapeutic Effect of Vitamin C in an Animal Model of Complex Regional Pain Syndrome Produced by Prolonged Hindpaw Ischemia-Reperfusion in Rats. International Journal of Medical Sciences 14(1): 97-101, 2017

Reflex sympathetic dystrophy syndrome (RSDS), complex regional pain syndrome-type 1 (CRPS 1), neuropathic pain: an equine perspective. Journal of equine veterinary science 16(11): 463-468, 1996

Anti-allodynic Effect of Mangiferin in Rats With Chronic Post-ischemia Pain: A Model of Complex Regional Pain Syndrome Type I. Frontiers in Pharmacology 9: 1119, 2018

Experimental pressure pain in patients with complex regional pain syndrome, Type I (reflex sympathetic dystrophy). American Journal of Physical Medicine and Rehabilitation 77(5): 382-387, 1998

A hypothesis for the cause of complex regional pain syndrome-type I (reflex sympathetic dystrophy): pain due to deep-tissue microvascular pathology. Pain Medicine 11(8): 1224-1238, 2011

Rats with chronic post-ischemia pain exhibit an analgesic sensitivity profile similar to human patients with complex regional pain syndrome--type I. European Journal of Pharmacology 583(1): 97-102, 2008

Health-related quality of life in chronic refractory reflex sympathetic dystrophy (complex regional pain syndrome type I). Journal of Pain and Symptom Management 20(1): 68-76, 2000

Complex regional pain syndrome type I (reflex sympathetic dystrophy): more than a myth. Neurology 51(1): 4-5, 1998

Role of NFkappaB in an animal model of complex regional pain syndrome-type I (CRPS-I). Journal of Pain 10(11): 1161-1169, 2010

Sympathetic reflex dystrophy/syndrome of regional pain complex type I Complementary explorations. Revista Espanola de Reumatologia 25(7): 270-275, Aug -Sept, 1998

Physical therapy in sympathetic reflex dystrophy/syndrome of regional pain complex type I. Revista Espanola de Reumatologia 25(7): 290-292, Aug -Sept, 1998

Patterns of spread in complex regional pain syndrome, type I (reflex sympathetic dystrophy). Pain 88(3): 259-266, 2000

Antiallodynic Effects of Bee Venom in an Animal Model of Complex Regional Pain Syndrome Type 1 (CRPS-I). Toxins 9(9), 2018

Current management of reflex sympathetic dystrophy syndrome (complex regional pain syndrome type I). Joint, Bone, Spine 73(5): 495-499, 2006

Thermal grill illusion and complex regional pain syndrome type I (reflex sympathetic dystrophy). Regional Anesthesia 22(3): 257-259, 1997