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Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein



Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein



Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae 25(5): 504-507



To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain. According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA. Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained. The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.

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Accession: 048565107

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PMID: 14650145


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