+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Complete nucleotide sequence and analysis of pPSR1 (72,601 bp), a pPT23A-family plasmid from Pseudomonas syringae pv. syringae A2



Complete nucleotide sequence and analysis of pPSR1 (72,601 bp), a pPT23A-family plasmid from Pseudomonas syringae pv. syringae A2



Molecular Genetics and Genomics 270(6): 462-476



Plasmid pPSR1 is a conjugative plasmid originally isolated from Pseudomonas syringae pv. syringae A2, and is a member of the recently described pPT23A plasmid family. We have determined the complete sequence of pPSR1 and found the plasmid to be 72,601 bp in length, encoding 55 ORFs. Putative functions were assigned to 49 ORFs; of these, 24 (49.0%) are involved in plasmid replication, maintenance or conjugation, 17 (34.7%) have roles in virulence or ecological fitness, and eight (16.3%) encode transposase functions as part of mobile elements. pPSR1 carries the effector gene orf34, the mutagenic DNA repair operon rulAB which confers tolerance to ultraviolet radiation, and two genes for methyl-accepting chemotaxis proteins, one of which was located within the novel transposon Tn 5395. The streptomycin resistance transposon Tn 5393a, which carries a strA-strB determinant, was found inserted immediately downstream of the pPSR1 repA gene. Functional analysis of the replication region of pPSR1 indicated that the repA gene and flanking upstream and downstream sequences are required for autonomous replication in P. syringae. Hybridization analyses of the distribution of 11 of the pPSR1 ORFs indicated that many of the ecologically important ORFs were confined to the pathovar P. syringae pv. syringae -either to strains from the local population from which pPSR1 was originally isolated, or strains from a worldwide collection. Conjugative transfer genes and a gene encoding a transcriptional regulator were more widely distributed among several P. syringae pathovars. The sequence analysis of pPSR1 suggests that pPT23A-family plasmids evolve by accumulating genes that are important for host-pathogen interactions or growth on plant hosts, which are incorporated onto a conserved backbone encoding conjugation and stability determinants.

(PDF emailed within 0-6 h: $19.90)

Accession: 048611323

Download citation: RISBibTeXText

PMID: 14634868

DOI: 10.1007/s00438-003-0945-9


Related references

Nucleotide sequence analysis of pPSR1, a Pseudomonas syringae pPT23A-family plasmid. Phytopathology 92(6 Supplement): S79, 2002

Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family. Bmc Genomics 18(1): 365-365, 2017

Phylogenetic analysis of the pPT23A plasmid family of Pseudomonas syringae. Applied and Environmental Microbiology 73(4): 1287-1295, 2006

Comparative genomic analysis of the pPT23A plasmid family of Pseudomonas syringae. Journal of Bacteriology 187(6): 2113-2126, 2005

Nucleotide sequence analysis of the iaa biosynthetic genes of pseudomonas syringae pv syringae. Phytopathology 81(10): 1245, 1991

A note: Serological study of four pathovars of Pseudomonas syringae: Pseudomonas syringae aptata, Pseudomonas syringae tabaci, Pseudomonas syringae mors prunorum and Pseudomonas syringae phaseolicola. Journal of Applied Bacteriology 74(6): 683-687, 1993

Plasmid dna relationships in plant pathogenic pseudomonas syringae pathovar tabaci pseudomonas syringae pathovar angulata pseudomonas syringae pathovar coronafaciens and pseudomonas syringae pathovar striafaciens. Abstracts of the Annual Meeting of the American Society for Microbiology 85: 134, 1985

Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle. Plos One 10(8): E0136101, 2016

Nucleotide sequence and properties of the hrmA locus associated with the Pseudomonas syringae pv. syringae 61 hrp gene cluster. Molecular Plant-Microbe Interactions 6(5): 553-564, 1993

Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola. Applied and Environmental Microbiology 64(9): 3180-3187, 1998

Pseudomonas syringae exchangeable effector loci: sequence diversity in representative pathovars and virulence function in P. syringae pv. syringae B728a. Journal of Bacteriology 185(8): 2592-2602, 2003

Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. Journal of Bacteriology 186(15): 5101-5115, 2004

Nucleotide sequence, functional characterization and evolution of pFKN, a virulence plasmid in Pseudomonas syringae pathovar maculicola. Molecular Microbiology 47(6): 1545-1562, 2003

Multilocus sequence typing of Pseudomonas syringae sensu lato confirms previously described genomospecies and permits rapid identification of P. syringae pv. coriandricola and P. syringae pv. apii causing bacterial leaf spot on parsley. Phytopathology 101(7): 847-858, 2012

Genetic and dna sequence analysis of the interval between two divergently transcribed phytotoxin biosynthesis genes from pseudomonas syringae pathovar syringae. Phytopathology 79(10): 1178-1179, 1989