+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Computerized morphometry of the cirrhotic liver: comparative analysis in primary biliary cirrhosis, alcoholic cirrhosis, and posthepatitic cirrhosis



Computerized morphometry of the cirrhotic liver: comparative analysis in primary biliary cirrhosis, alcoholic cirrhosis, and posthepatitic cirrhosis



Microscopy Research and Technique 65(3): 113-121



Fibrosis and nodular regeneration are the hallmarks of liver cirrhosis. To assess the degree of fibrosis and the severity of the structural changes affecting parenchymal and extraparenchymal components in liver cirrhosis, a computerized morphometric model has been applied to liver specimens from patients undergoing liver transplantation for primary biliary cirrhosis, posthepatitic and alcoholic cirrhosis. Fifty-eight hepatectomy specimens from patients undergoing liver transplantation for cirrhosis were analyzed: 17 alcoholic, 28 posthepatitic (HBV-related and HCV-related cirrhosis), and 13 primary biliary cirrhoses. Liver specimens were fixed in 10% neutral-buffered formalin and embedded in paraffin. Sections were stained with chromotrope-aniline blue method and monoclonal antibodies against cytokeratin 7 and CD31. Volume fractions of parenchymal compartment and fibrosis were stereologically determined on the specimens stained with chromotrope-aniline blue method. Volume fractions of portal bile ducts, proliferated bile ductules, and hepatocytes with biliary metaplasia were measured on cytokeratin 7 stains, while volume fractions of capillary units have been evaluated on CD31 staining. Volume fraction of fibrosis was higher in primary biliary cirrhosis than in the other disease-induced cirrhosis. The main differences were related to immunohistochemical staining. Volume fraction of hepatocytes with biliary metaplasia was higher in HCV-related cirrhosis, whereas volume fractions of biliary structures were more prominent in HBV-related cirrhosis. Primary biliary cirrhosis was characterized by a reduced number of bile ducts and by a wider expression of cytokeratin 7 into periportal hepatocytes. Capillary units were more prominent in primary biliary cirrhosis than alcoholic and posthepatitic cirrhosis. Our computerized morphometric model well describes and quantifies the morphological alterations of the liver and it could represent an adjunctive tool to evaluate the degree of dysplastic phenomena involving parenchymal and extraparenchymal compartments.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048619074

Download citation: RISBibTeXText

PMID: 15605407

DOI: 10.1002/jemt.20110


Related references

Regenerative pattern of liver cells in primary biliary cirrhosis, alcoholic cirrhosis, posthepatitic cirrhosis (HBV-related) and hepatocellular carcinoma: Comparative analysis by computerized morphometry. Pathology International 46(4): 267-273, 1996

A comparative study of liver cell regenerative activity between primary biliary cirrhosis, alcoholic cirrhosis, hepatitic cirrhosis, and hepatocellular carcinoma using figure analysis. Tropical Medicine 37(1): 13-20, 1995

Analysis of histological and immunohistochemical patterns of the liver in posthepatitic and alcoholic cirrhosis by computerized morphometry. Modern Pathology 15(8): 798-806, 2002

Spontaneous regression of oesophageal varices after long-term conservative treatment. Retrospective study in 20 patients with alcoholic liver cirrhosis, posthepatitic cirrhosis and haemochromatosis with cirrhosis. Journal of Hepatology 10(2): 158-162, 1990

Posthepatitic cirrhosis clinically simulating extrahepatic biliary obstruction (so-called "primary biliary cirrhosis"). New England Journal of Medicine 268: 629-639, 1963

A case of hepatocellular carcinoma developed in a patient with alcoholic liver cirrhosis with primary biliary cirrhosis. Nihon Shokakibyo Gakkai Zasshi 97(1): 54-59, 2000

Serum prealbumin, transferrin and immunoglobulins in fatty liver, alcoholic cirrhosis and primary biliary cirrhosis. Clinica Chimica Acta; International Journal of Clinical Chemistry 129(3): 279-286, 1983

Coronary Artery Disease in Liver Cirrhosis: A Comparison Between Nash-Related Cirrhosis, Alcoholic Cirrhosis and Cirrhosis Due to Other Etiologies. Gastroenterology 136(5): A-796, 2009

Serum free thyroid hormone concentrations and indices in alcoholic liver cirrhosis, primary biliary cirrhosis and chronic active hepatitis. Acta Endocrinologica. Supplementum 251: 21-26, 1983

Expression and DNA-binding activity of signal transducer and activator of transcription 3 in alcoholic cirrhosis compared to normal liver and primary biliary cirrhosis in humans. American Journal of Pathology 162(2): 587-596, 2003

Non invasive doppler us evaluation of portal flow in posthepatitic hc alcoholic ac and primary biliary pbc cirrhosis. Journal of Hepatology 9(Suppl. 1): S117, 1989

Characteristic differences according to the cirrhotic pattern of advanced primary biliary cirrhosis: Macronodular cirrhosis indicates slow progression. Hepatology Research 36(3): 188-194, 2006

Liver cirrhosis.An advance on diagnosis and treatment.4.Clinical feature of alcoholic cirrhosis.The comparison with viral liver cirrhosis. Nihon Naika Gakkai Zasshi 80(10): 1587-1591, 1991

Clinicopathological studies and operative results of hepatocellular carcinoma with liver cirrhosis, comparing HB-associated cirrhosis to alcoholic and post-transfusion cirrhosis. Gastroenterologia Japonica 25(1): 54-60, 1990

Duplex doppler study of portal hemodynamics in primary biliary cirrhosis pbc comparison with alcoholic and viral hepatitis related cirrhosis. Gastroenterology 96(5 Part 2): A578, 1989