EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Construction of VEGF recombinant plasmid pcDNA/V and its expression in model rats with acute myocardial ischemia


Construction of VEGF recombinant plasmid pcDNA/V and its expression in model rats with acute myocardial ischemia



Sheng Wu Gong Cheng Xue Bao 22(2): 220-225



ISSN/ISBN: 1000-3061

PMID: 16607947

The cDNA encoding human Vascular Endothelial Growth Factor 165 (VEGF165) was amplified using RT-PCR from human tonsil tissue and cloned into eukaryotic expression vector pcDNA3.1 (+). The recombinant plasmid pcDNA/V was transferred into 293 cells mediated by liposome and the cells stably expressing VEGF were selected under the pressure of G418. ELISA and Western blotting demonstrated that the eukaryotic expression vector pcDNA/V was successfully constructed and its corresponding protein could be expressed efficiently in vitro. Chick Charioallantoic Membrane (CAM) bioassay showed that recombinant protein has biological activity of hVEGF. Model rats with acute myocardial ischemia were used to further study the expression of VEGFin vivo. The model rats were divided randomly into three groups: control group, pcDNA3.1 (+) group and pcDNA/V group. 50microL naked plasmid DNA or saline was intramyocardially injected at three sites into the border zone of infarction. The hearts of rats were excised and fixed histologically, then the infarction sizes were studied by immunohistochemical staining and electron microscope after four weeks. Immunohistochemical staining for VEGF appeared to be negative in control and pcDNA3.1 (+) groups. In pcDNA/V group, myocardial cells in infarction border zone showed positive staining for VEGF in cytoplasm. Ultrastructural anaylsis showed that there were visible hyperplasia of vascular endothilium in pcDNA/V group. The control and pcDNA3.1 (+) groups showed less capillary hyperplasia. In this study, VEGF165 gene was successfully cloned and its protein expressed in vitro and in vivo was of bioactivity, which provides a basis for the further study of biological functions of human VEGF.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 048641871

Download citation: RISBibTeXText

Related references

Construction of eukaryotic expression recombinant plasmid PCDNA-3-SJ26 of Schistosoma japonicum and its expression in dendritic cell. Jishengchong Yu Yixue Kunchong Xuebao ; 12(2): 65-70, 2005

An experimental study on use of 7T MRI for evaluation of myocardial infarction in SD rats transfected with pcDNA 3.1(+)/VEGF121 plasmid. American Journal of Translational Research 8(8): 3376-3386, 2016

VEGF and VEGF receptors expression in acute myocardial infarction model of angiogenesis. Circulation 92(8 Suppl. ): I713, 1995

Construction of sequencing recombinant plasmid and eukaryotic expression recombinant plasmid of CTP (phosphocholine cytidylytransferase) from Plasmodium falciparum (FCC1/HN). Chinese Journal of Zoonoses 18(3): 22-24, 2002

Construction of a recombinant Pim3-expressing plasmid and expression and activity thereof: experiment with rats. Zhonghua Yi Xue Za Zhi 87(36): 2567-2570, 2007

Enhancement of DNA vaccine (P12A3C-pcDNA) efficacy against foot-and-mouth disease by coadministration of interleukin-18-expressing (IL18 pcDNA) plasmid in guinea-pigs. Fems Immunology and Medical Microbiology 60(3): 261-269, 2010

Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia-reperfusion model. Journal of Controlled Release: Official Journal of the Controlled Release Society 147(1): 30-37, 2010

Cathepsin L expression in plasma after acute myocardial ischemia and ischemia-reperfusion in rats. Fa Yi Xue Za Zhi 30(4): 253-256, 2014

Expression and significance of PAI-1, u-PA, VEGF and CD34 in acute cerebral ischemia rats based on atherosclerosis. Wuhan Daxue Xuebao (Yixue Ban) 28(6): 711-714, 2007

Myocardial angiogenesis after plasmid or adenoviral VEGF-A(165) gene transfer in rat myocardial infarction model. Cardiovascular Research 73(3): 481-487, 2007

Post-translational regulation of a hypoxia-responsive VEGF plasmid for the treatment of myocardial ischemia. Biomaterials 34(26): 6229-6238, 2013

Effects of ginsenoside Rg1 on angiogenesis and VEGF expression in rats with acute myocardial infarction. Journal of China Medical University 36(5): 517-519, 2007

The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression. Bmc Psychiatry 14: 349, 2014

Effects of electroacupuncture at different acupoints on apoptosis and the expression of mi RNAs in myocardial cells in rats model of myocardial ischemia. Zhongguo Zhen Jiu 36(3): 281-286, 2016

Enhanced expression of VEGF mRNA and angiogenic activity after acute myocardial infarction in rats. Journal of Molecular and Cellular Cardiology 30(6): A42, 1998