+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus reverse genetics and development of vectors for gene expression



Coronavirus reverse genetics and development of vectors for gene expression



Current Topics in Microbiology and Immunology 287: 161-197



Knowledge of coronavirus replication, transcription, and virus-host interaction has been recently improved by engineering of coronavirus infectious cDNAs. With the transmissible gastroenteritis virus (TGEV) genome the efficient (>40 microg per 106 cells) and stable (>20 passages) expression of the foreign genes has been shown. Knowledge of the transcription mechanism in coronaviruses has been significantly increased, making possible the fine regulation of foreign gene expression. A new family of vectors based on single coronavirus genomes, in which essential genes have been deleted, has emerged including replication-competent, propagation-deficient vectors. Vector biosafety is being increased by relocating the RNA packaging signal to the position previously occupied by deleted essential genes, to prevent the rescue of fully competent viruses that might arise from recombination events with wild-type field coronaviruses. The large cloning capacity of coronaviruses (>5 kb) and the possibility of engineering the tissue and species tropism to target expression to different organs and animal species, including humans, has increased the potential of coronaviruses as vectors for vaccine development and, possibly, gene therapy.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 048667175

Download citation: RISBibTeXText

PMID: 15609512


Related references

Construction of SARS coronavirus partial S gene segments from eukaryotic expression vectors and its expression in Hela cells. Applied Immunohistochemistry & Molecular Morphology 11(4): 296, 2003

Coronavirus replication and reverse genetics. 2005

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development. Journal of Virological Methods 160(1-2): 48-56, 2009

Coronavirus reverse genetics by targeted RNA recombination. Current Topics in Microbiology and Immunology 287: 133-159, 2005

Reverse genetics system for the avian coronavirus infectious bronchitis virus. Journal of Virology 75(24): 12359-12369, 2001

Chloroplast gene expression and reverse genetics in Chlamydomonas reinhardtii. NATO ASI series: Series A: Life sciences26(226): 9-16, 1992

Reverse genetics of SARS-related coronavirus using vaccinia virus-based recombination. Plos one 7(3): E32857, 2012

Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets. Journal of Clinical Microbiology 43(5): 2041-2046, 2005

Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. Proceedings of the National Academy of Sciences of the United States of America 110(40): 16157-16162, 2013

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus. Proceedings of the National Academy of Sciences of the United States of America 100(22): 12995-13000, 2003

Reverse genetics of coronaviruses using vaccinia virus vectors. Current Topics in Microbiology and Immunology 287: 199-227, 2005

The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host. Virology 296(1): 177-189, 2002

Reverse genetics and transient gene expression in fleshy fruits: overcoming plant stable transformation. Plant Signaling and Behavior 4(9): 864-867, 2009

Development of murine leukemia virus-based self-activating vectors that efficiently delete the selectable drug resistance gene during reverse transcription. Journal of Virology 73(10): 8837-8842, 1999

Reverse genetics in the tide pool: knock-down of target gene expression via RNA interference in the copepod Tigriopus californicus. Molecular Ecology Resources 15(4): 868-879, 2015