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Deoxyribosyl-8-Azaadenine. its Deamination to Deoxyribosyl-8-Azahypoxanthine and effect on Nucleic Acid Synthesis in Ehrlich Ascites cells in Vitro

Frederiksen, S.

Biochimica et Biophysica Acta 87: 574-582

1964

ISSN/ISBN: 0006-3002
PMID: 14220687
DOI: 10.1016/0926-6550(64)90275-0
Accession: 048734560
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Deoxyribosyl-8-azaadenine has been prepared by deoxyribose transfer from thymidine or deoxyinosine to 8-azaadenine with nucleoside deoxyribosyltransferase (Ec 2.4.2.6) from Lactobacillus helveticus. Deoxyribosyl-8-azahypoxanthine was prepared by enzymatic deamination of deoxyribosyl-8-azaadenine. Deoxyribosyl-8-azaadenine is substrate for adenosine deaminase (Ec 3.5.4.4) with a Km value of 125·10−6 and a vmax about 4 times that of deoxyadenosine. Deoxyribosyl-8-azaadenien and deoxyribosyl-8-azahypoxanthine have no effect on deoxyribonucleic acid and ribonucleic acid synthesis in Ehrlich ascites cells in vitro. Deoxyribosyl-8-azaadenine is rapidly deaminated in the tumor cells, but 8-azaadenine-deoxyribotides could not be detected. Deoxyribosyl-8-azahypoxanthine is neither cleaved in Ehrlich cells nor by purine nucleoside phosphorylase (Ec 2.4.2.1).

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Related References

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