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Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay



Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay



Di 1 Jun Yi Da Xue Xue Bao 25(4): 429



To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA). Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit. The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%;-6.2%;, and coefficient of variability between days of 5.3%;-9.6%;. The results of detection were consistent between ELISA and TRFIA. TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.

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Accession: 048744455

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PMID: 15837647


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