EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Disulfide bridge disruption in the alpha2 domain of the HLA class i molecule leads to low expression of the corresponding antigen


Disulfide bridge disruption in the alpha2 domain of the HLA class i molecule leads to low expression of the corresponding antigen



Human Immunology 67(8): 589-596



ISSN/ISBN: 0198-8859

PMID: 16916654

DOI: 10.1016/j.humimm.2006.04.010

Using sequence-based typing, we have identified a novel human leukocyte antigen (HLA)-A*30 allele, HLA-A*3014L, with a low expression pattern. The sequence of HLA-A*3014L is identical to that of HLA-A*3001 except for a G to C substitution in exon 3 at nucleotide position 563, resulting in an amino acid difference at position 164 (Cys to Ser). Due to the cysteine substitution, a disulfide bridge in the alpha2 domain of the HLA class I heavy chain cannot be formed. By using the standard microlymphocytotoxicity test, the HLA-A30 antigen cannot be detected. By flow cytometric analysis of the cell-surface expression at either 37 degrees C or 30 degrees C, a temperature-sensitive expression pattern of the HLA-A*3014L antigen was observed. Only by incubating the cells at 30 degrees C, which increases the stability of HLA class I heavy chains, was a weak but clearly detectable HLA-A*3014L expression found. The mRNA expression level of the HLA-A*3014L allele was not affected by the nucleotide substitution. The intrachain disulfide bond formation in the alpha2 domain is essential for the normal expression of the HLA molecules. Reduced protein expression is probably caused by incorrect HLA class I heavy chain folding and HLA class I complex assembly.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048815223

Download citation: RISBibTeXText

Related references

Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen. Proceedings of the National Academy of Sciences of the United States of America 83(3): 757-761, 1986

Mutation of the alpha 2 domain disulfide bridge of the class I molecule HLA-A*0201. Effect on maturation and peptide presentation. Human Immunology 39(4): 261-271, 1994

A novel HLA-A*30 null allele with disulfide bridge disruption in the alpha 2 domain. Human Immunology 64(Suppl 1): S157, 2003

Abrogation of tumorigenicity by MHC class Ii antigen expression requires the cytoplasmic domain of the class Ii molecule. Journal of Immunology 147(7): 2419-2422, 1991

Abrogation of tumorigenicity by MHC class II antigen expression requires the cytoplasmic domain of the class II molecule. The Journal of Immunology (1950) 147(7): 2419-2422, 1991

Antigen recognition by the T cell receptor is enhanced by CD8 alpha-chain binding to the alpha 3 domain of MHC class i molecules, not by signaling via the cytoplasmic domain of CD8 alpha. International Immunology 4(2): 147-152, 1992

Amino terminal half of the alpha-1 domain of the mouse L-d class I molecule determines its assembly and surface expression. Journal of Immunology 150(8 Part 2): 119A, 1993

A polymorphic residue in the amino terminal alpha 1 hemi-domain of the mouse Ld class i molecule affects its assembly and surface expression. Molecular Immunology 31(15): 1169-1180, 1994

A single disulfide bridge is crucial for alpha-dystroglycan N-terminal domain stability. Fleischmajer, R, Timpl, R, Werb, Z Annals of the New York Academy of Sciences; Morphogenesis: Cellular interactions 228-231, 1998

Role of a disulfide bridge in the immune function of major histocompatibility class i antigen as studied by in vitro mutagenesis. Proceedings of the National Academy of Sciences of the United States of America 81(23): 7544-7548, 1984

The thiol groups of mouse immunoglobulin A. Incomplete formation of the C alpha 1-domain disulphide bridge. Biochemical Journal 225(1): 113-125, 1985

Identification of a disulfide bridge connecting the alpha-subunits of the extracellular domain of the insulin receptor. Biochemical and Biophysical Research Communications 189(2): 650-653, 1992

A single disulfide bridge (Cys182-Cys264) is crucial for alpha-dystroglycan N-terminal domain stability. Annals of the new York Academy of Sciences 857: 228-231, 1998

Recognition by CD8 on cytotoxic T lymphocytes is ablated by several substitutions in the class i alpha 3 domain: CD8 and the T-cell receptor recognize the same class i molecule. Proceedings of the National Academy of Sciences of the United States of America 87(6): 2137-2141, 1990

Allo-class I-reactive CD4-CD8- T cell hybridomas recognize the conformational change of class i molecule resulting from the exchange of the alpha 3 domain. European Journal of Immunology 19(10): 1861-1865, 1989