+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

EGF/PI3K signaling pathway regulates the expression of osteopontin in liver cancer HepG2 cells

EGF/PI3K signaling pathway regulates the expression of osteopontin in liver cancer HepG2 cells

Zhonghua Yi Xue Za Zhi 83(22): 1980-1983

To investigate the effect of epidermal growth factor (EGF) on the expression of osteopontin (OPN) in liver cancer HepG2 cells. Human liver caner cells of HepG2 line were cultured in medium without bovine serum. EGF was added. Another cells were pretreated with specific phosphatidylinositol 3 (PI3) inhibitor Wortmannin and then added with EGF. HepG2 cell were collected. RNase prote4ction assay and Western blotting were used to examine the expression of OPN in the HepG2 cells. HepG2 cells cultured in the bovine serum free medium expressed very low level of OPN. EGF increased the expression of OPN mRNA and protein in a dose- and time-dependent manners. Pretreatment of Wortmannin significantly reduced the EGF-induced OPN expression. EGF increases the OPN expression, possibly through PI3 signaling pathway. It is possible to block the synthesis of OPN through manipulating several target genes, thus inhibiting the metastasis of liver cancer.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 048845784

Download citation: RISBibTeXText

PMID: 14703434

Related references

Insulin/PI3K signalling pathway regulates the expression of survivin in liver cancer HepG2 cells. Zhonghua Zhong Liu Za Zhi 30(10): 745-748, 2010

Gigantol attenuates the proliferation of human liver cancer HepG2 cells through the PI3K/Akt/NF-κB signaling pathway. Oncology Reports 37(2): 865-870, 2016

EGFR signaling pathway negatively regulates PSA expression and secretion via the PI3K-Akt pathway in LNCaP prostate cancer cells. Biochemical and Biophysical Research Communications 342(1): 92-100, 2006

Capn4 regulates migration and invasion of ovarian carcinoma cells via targeting osteopontin-mediated PI3K/AKT signaling pathway. Oncology Letters 17(1): 564-570, 2019

TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells. Tumour Biology 37(3): 3675-3681, 2017

Enhancement of osteopontin expression in HepG2 cells by epidermal growth factor via phosphatidylinositol 3-kinase signaling pathway. World Journal of Gastroenterology 10(2): 205-208, 2004

Isoorientin induces apoptosis through mitochondrial dysfunction and inhibition of PI3K/Akt signaling pathway in HepG2 cancer cells. Toxicology and Applied Pharmacology 265(1): 83-92, 2013

Staurosporine suppresses survival of HepG2 cancer cells through Omi/HtrA2-mediated inhibition of PI3K/Akt signaling pathway. Tumour Biology 39(3): 1010428317694317, 2017

Furazolidone induces apoptosis through activating reactive oxygen species-dependent mitochondrial signaling pathway and suppressing PI3K/Akt signaling pathway in HepG2 cells. Food and Chemical Toxicology 75: 173-186, 2015

Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells. International Journal of Molecular Medicine 2019, 2019

GRK2 negatively regulates IGF-1R signaling pathway and cyclins' expression in HepG2 cells. Journal of Cellular Physiology 228(9): 1897-1901, 2013

FFJ-3 inhibits PKM2 protein expression via the PI3K/Akt signaling pathway and activates the mitochondrial apoptosis signaling pathway in human cancer cells. Oncology Letters 13(4): 2607-2614, 2017

IGF-PI3K-AKT signaling pathway regulates myogenin expression in normal myogenic cells but not in rhabdomyosarcoma cells. Cell Biology International 24(12): 934, 2000

Anticancer effect of calycopterin via PI3K/Akt and MAPK signaling pathways, ROS-mediated pathway and mitochondrial dysfunction in hepatoblastoma cancer (HepG2) cells. Molecular and Cellular Biochemistry 397(1-2): 17-31, 2015

IL-6 regulates Mcl-1L expression through the JAK/PI3K/Akt/CREB signaling pathway in hepatocytes: implication of an anti-apoptotic role during liver regeneration. Plos One 8(6): E66268, 2014