EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of ELISA kits for detection of Mycoplasma pneumoniae--specific IgG, IgA, IgM antibodies on the diagnosis of Mycoplasma pneumoniae infection in children


Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 79(7): 457-463
Evaluation of ELISA kits for detection of Mycoplasma pneumoniae--specific IgG, IgA, IgM antibodies on the diagnosis of Mycoplasma pneumoniae infection in children
A retrospective study was conducted to evaluate the utility of Mycoplasma pneumoniae IgG (quantitative), IgA (quantitative), IgM (qualitative) ELISA kits (Medac Diagnostika, Germany) for the diagnosis of M. pneumoniae pneumonia in children under 16 years of age. This study included a total of 159 serum samples from 113 patients with acute respiratory diseases such as bronchitis, pneumonia, which were classified into three groups according to the results of a particle agglutination (PA) test as a reference method, that is, Group I (Mycoplasma-definite cases): Group I-a (paired 52 samples from 26 cases); a four-fold or greater rise of antibody from an acute phase PA titer of < or = 1:80, Group I-b (paired 12 samples from 6 cases); a four-fold or greater rise of antibody from an acute phase PA titer of > or = 1:160, Group I-c (48 samples from 38 cases); a single high PA titer of > or = 1:640 either or both in acute or convalescent serum, Group II (Mycoplasma-probable cases, 18 samples from 17 cases): a PA titer of 1:160 or 320 was observed either or both in acute or convalescent serum, but the above serological criteria for Group I were not fulfilled, Group III (non-cases, 29 samples from 26 cases): a PA titer of any sample was < or = 1:80. The ELISA tests were performed according to the supplier's recommendations, and the results were classified according to the interpretation provided by the supplier: Early stage of infection (category 1,2), Acute- (3,4,5), Current- (6), Past- (7), and No-infection (8). The day of onset of fever (defined as a body temperature of > or = 37.5 degrees Celsius) was denoted as day 0. As a result from Group I, the category initially observed following the onset of fever was category 8 (triple negative), and the predominance of category 8 was replaced by category 1 (IgM solely positive) after day 4, followed by a shift of predominance to category 4 (IgM and IgG double positive) or 5 (triple positive) after day 10 or later. Specifically, category 1 was rather exclusively observed before day 21 following the onset of fever. These results suggest that category 1, when observed, is a useful marker of acute infection by Mycoplasma pneumoniae in children because it appears early in the acute phase and no longer observed beyond the convalescent phase. On the other hand, significance of detecting IgA antibody, which must be important for adults, was not remarkable in our study. Five samples in group II and 3 samples in group III fell into category 1. Whether or not such cases, in the absence of significant PA titers, can be taken actually as mycoplasmal infection remains to be clear. This study validated the utility of this ELISA methodology in terms of the acute phase diagnosis using a single point serum sample for Mycoplasma pneumoniae infection specifically in children.

(PDF 0-2 workdays service: $29.90)

Accession: 048992854

PMID: 16119804



Related references

Laboratory diagnosis of mycoplasma pneumoniae infection 3. detection of igm antibodies to mycoplasma pneumoniae by a modified indirect hemagglutination test. Epidemiology and Infection 103(3): 613-624, 1989

Evaluation of humoral response to Mycoplasma pneumoniae antigens in natural infection in human using conventional and new generation tests. III. ELISA, complement fixation and immunoelectroprecipitation tests in diagnosis of Mycoplasma pneumoniae infections: Comparative analysis. Medycyna Doswiadczalna i Mikrobiologia 47(1-2): 77-88, 1995

Evaluation of conventional and new generation tests for testing the humeral response to Mycoplasma pneumoniae antigens in natural infection in humans. III Use of ELISA, complement fixation and immunoelectroprecipitation tests in diagnosis of Mycoplasma pneumoniae infections--comparative analysis. Medycyna Doswiadczalna i Mikrobiologia 47(1-2): 77-88, 1995

Laboratory diagnosis of Mycoplasma pneumoniae infection. 3. Detection of IgM antibodies to M. pneumoniae by a modified indirect haemagglutination test. Epidemiology and Infection 103(3): 613-623, 1989

A new elisa for the detection of mycoplasma pneumoniae specific antibodies. American Review of Respiratory Disease 145(4 PART 2): A540, 1992

Occurrence of mycoplasma antibodies in children mycoplasma pneumoniae mycoplasma salivarium mycoplasma hominis i mycoplasma laidlawii. Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Abteilung I Originale 208(1-2): 289-294, 1968

Detection of specific IgM antibodies for the diagnosis of Mycoplasma pneumoniae infections: a clinical evaluation. Scandinavian Journal of Infectious Diseases 20(6): 601-610, 1988

Comparison of IgM specific commercial enzyme immunoassay kits for the serologic diagnosis of Mycoplasma pneumoniae infection. Abstracts of the General Meeting of the American Society for Microbiology 101: 390, 2001

Mycoplasma pneumoniae--stimulation of lymphocytes obtained from adenoid vegetations and blood in children with and without serological evidence of Mycoplasma pneumoniae infection. Acta Pathologica, Microbiologica, et Immunologica Scandinavica. Section C, Immunology 92(5): 313-317, 1984

A study of Mycoplasma pneumoniae infection and combined Mycoplasma pneumoniae and viral infections in adults and children. Voprosy Virusologii 13(6): 674-681, 1968