+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming

Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming

Methods in Molecular Biology 1733: 255-263

MicroRNAs are small 18-24 nt single-stranded noncoding RNA molecules involved in many biological processes, including stemness maintenance and cellular reprogramming. Current methods used in loss-of-function studies of microRNAs have several limitations. Here, we describe a new approach for dissecting miR-302/367 functions by transcription activator-like effectors (TALEs), which are natural effector proteins secreted by Xanthomonas and Ralstonia bacteria. Knockdown of the miR-302/367 cluster uses the Kruppel-associated box repressor domain fused with specific TALEs designed to bind the miR-302/367 cluster promoter. Knockout of the miR-302/367 cluster uses two pairs of TALE nucleases (TALENs) to delete the miR-302/367 cluster in human primary cells. Together, both TALE-based transcriptional repressor and TALENs are two promising approaches for loss-of-function studies of microRNA cluster in human primary cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 048993037

Download citation: RISBibTeXText

PMID: 29435939

DOI: 10.1007/978-1-4939-7601-0_21

Related references

Dissecting the roles of miR-302/367 cluster in cellular reprogramming using TALE-based repressor and TALEN. Stem Cell Reports 1(3): 218-225, 2013

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency. Cell Regeneration 5: 2, 2016

Cellular reprogramming technology for dissecting cancer epigenome in vivo. Epigenomics 9(7): 997, 2017

MicroRNA Regulation Along the Course of Cellular Reprogramming to Pluripotency. Current Molecular Medicine 18(1): 58-64, 2018

Identification of Cellular Targets of MicroRNA-181a in HepG2 Cells: A New Approach for Functional Analysis of MicroRNAs. Plos one 10(4): E0123167, 2015

Inferring the human microRNA functional similarity and functional network based on microRNA-associated diseases. Bioinformatics 26(13): 1644-1650, 2010

MicroRNA-based cancer cell reprogramming technology. Experimental and Therapeutic Medicine 4(1): 8, 2012

MicroRNA 302/367 Cluster Effectively Facilitates Direct Reprogramming from Human Fibroblasts into Functional Neurons. Stem Cells and Development 24(23): 2746-2755, 2015

MicroRNA-based system in stem cell reprogramming; differentiation/dedifferentiation. International Journal of Biochemistry and Cell Biology 55: 318-328, 2014

Systems biology approach to identify transcriptome reprogramming and candidate microRNA targets during the progression of polycystic kidney disease. Bmc Systems Biology 5: 56, 2011

Cellular reprogramming: a new approach to modelling Parkinson's disease. Biochemical Society Transactions 40(5): 1152-1157, 2012

High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays. Methods in Molecular Biology 1039: 261-277, 2013

MiRAW: A deep learning-based approach to predict microRNA targets by analyzing whole microRNA transcripts. Plos Computational Biology 14(7): E1006185, 2018

Dissecting microRNA biogenesis and microRNA-mediated regulation of gene network. Seikagaku. Journal of Japanese Biochemical Society 87(4): 413-421, 2015

The microRNA body map: dissecting microRNA function through integrative genomics. Nucleic Acids Research 39(20): E136, 2011