+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of VITEK 2 for analysis of Enterobacteriaceae using the Advanced Expert System (AES) versus interpretive susceptibility guidelines used at Dynacare Kasper Medical Laboratories, Edmonton, Alberta



Evaluation of VITEK 2 for analysis of Enterobacteriaceae using the Advanced Expert System (AES) versus interpretive susceptibility guidelines used at Dynacare Kasper Medical Laboratories, Edmonton, Alberta



Clinical Microbiology and Infection 9(11): 1091-1103



To evaluate the applicability and adaptability of the bioMérieux VITEK 2 Advanced Expert System (AES) to the customized interpretive susceptibility guidelines used at Dynacare Kasper Medical Laboratories (DKML). Three hundred isolates of Enterobacteriaceae (not more than 30% Escherichia coli) were tested on the VITEK 2 system and the API 20E for identification. Susceptibility testing for these isolates was performed on the VITEK 2 system and the Pasco broth microdilution panels. Minimal inhibitory concentrations (MICs) and interpreted results according to the AES and DKML antimicrobial susceptibility guidelines were compared. Of 300 isolates tested for susceptibility, 13 did not give AES interpretations. Of the remaining 287 isolates, interpretations between AES and DKML guidelines were compared for 10 antibiotics. The overall correlation between the AES and DKML interpretations was 96.2%. This study demonstrated the benefits and limitations of the bioMérieux AES. Automated knowledge-based systems provide a useful laboratory tool for the interpretation of susceptibility results.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048993156

Download citation: RISBibTeXText

PMID: 14616725

DOI: 10.1046/j.1469-0691.2003.00697.x


Related references

Multicentre evaluation of the VITEK 2 Advanced Expert System for interpretive reading of antimicrobial resistance tests. Journal of Antimicrobial ChemoTherapy 49(2): 289-300, 2002

Evaluation of the VITEK 2 advanced expert system for reporting piperacillin susceptibility in Klebsiella spp. Antimicrobial Agents and ChemoTherapy 52(6): 2291-2292, 2008

Laboratory-based evaluation of the colorimetric VITEK-2 Compact system for species identification and of the Advanced Expert System for detection of antimicrobial resistances: VITEK-2 Compact system identification and antimicrobial susceptibility testing. Diagnostic Microbiology and Infectious Disease 58(2): 191-198, 2007

Multicenter evaluation of the VITEK2 advanced expert system for interpretive reading of antimicrobial susceptibility data. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 40: 157, 2000

R2146 Detection of extended-spectrum -lactamases, including the Ctx-M-type enzymes, among Enterobacteriaceae using Vitek 2 system and the Advanced Expert system. International Journal of Antimicrobial Agents 29: S620-S621, 2007

Ability of the VITEK 2 advanced expert system To identify beta-lactam phenotypes in isolates of Enterobacteriaceae and Pseudomonas aeruginosa. Journal of Clinical Microbiology 38(2): 570-574, 2000

Multicenter laboratory evaluation of the bioMerieux Vitek antimicrobial susceptibility testing system with 11 antimicrobial agents versus members of the family Enterobacteriaceae and Pseudomonas aeruginosa. Journal of Clinical Microbiology 35(8): 2115-2119, 1997

Reevaluation of VITEK 2 susceptibility limitations after review by the Advanced Expert System. Abstracts of the General Meeting of the American Society for Microbiology 102: 122, 2002

Evaluation of the bioMerieux-Vitek Expert System for susceptibility tests. Abstracts of the General Meeting of the American Society for Microbiology 98: 211, 1998

Comparative evaluation of the VITEK 2 Advanced Expert System (AES) in five UK hospitals. Journal of Antimicrobial ChemoTherapy 51(5): 1191-1202, 2003

Performance of Vitek 2 for antimicrobial susceptibility testing of Enterobacteriaceae with Vitek 2 (2009 FDA) and 2014 CLSI breakpoints. Journal of Clinical Microbiology 53(3): 816-823, 2015

Comparative study of Vitek-2™ AIX versus Vitek-2™ PC: phenotypic resistance of enterobacteriaceae to β-lactams. Pathologie-Biologie 60(2): 78-83, 2012

Accurate characterization of ofloxacin susceptibility with Enterobacteriaceae using a modified GNS F6 card and the bioMerieux Vitek System. Diagnostic Microbiology & Infectious Disease. 25(3): 133-135, 1996

The Vitek 2 rapid susceptibility test system was evaluated for testing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp against cephalosporins. Abstracts of the General Meeting of the American Society for Microbiology 98: 137, 1998

Potential impact of the VITEK 2 system and the Advanced Expert System on the clinical laboratory of a university-based hospital. Journal of Clinical Microbiology 39(7): 2379-2385, 2001