+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of a panel of tumor markers for molecular detection of circulating cancer cells in women with suspected breast cancer



Evaluation of a panel of tumor markers for molecular detection of circulating cancer cells in women with suspected breast cancer



Clinical Cancer Research 11(10): 3722-3732



We examined the feasibility of using molecular characterization of circulating tumor cells as a method for early detection of breast cancer. Women without a prior history of cancer who had a breast abnormality detected on imaging followed by a breast biopsy were enrolled in this study. Density gradient centrifugation and immunomagnetic capture were used to enrich for epithelial cells from approximately 20 mL of blood. Real-time reverse transcription-PCR was used to quantitate the expression levels of the highly breast-specific genes, mammaglobin, gamma-aminobutyric acid type A receptor pi subunit (GABA A(pi)), B305D-C, and B726P in the epithelial cell-enriched samples. The assay was technically feasible in 154 of 199 accrued patients. From their clinical assessment, 100 patients had benign breast disease, 10 patients had ductal carcinoma in situ, and 44 patients had invasive breast cancer. We constructed a diagnostic test that classified patients with mammaglobin levels of at least 32.2 copies/pg beta-actin (units) in their circulating epithelial cells as positive for invasive breast cancer. This resulted in a sensitivity and specificity of 63.3% and 75.0%, respectively. A diagnostic test that classified patients as positive for invasive breast cancer when either mammaglobin levels were >46.3 units or B305D-C levels were >11.6 units increased the sensitivity and specificity to 70.5% and 81.0%, respectively. In the latter test, 12 of the 14 node-positive breast cancer patients were correctly identified. Including GABA A(pi) and B726P in the test did not increase its diagnostic potential. These results suggest that molecular characterization of circulating epithelial cells using mammaglobin and B305D-C offers potential for early detection of invasive breast cancer.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 048993805

Download citation: RISBibTeXText

PMID: 15897569

DOI: 10.1158/1078-0432.ccr-04-1483


Related references

Evaluation of proliferation and apoptosis markers in circulating tumor cells of women with early breast cancer who are candidates for tumor dormancy. Breast Cancer Research 16(6): 485, 2014

Clinical evaluation of a panel of mRNA markers in the detection of disseminated tumor cells in patients with operable breast cancer. Oncology Reports 14(2): 537-545, 2005

Diagnostic yield of primary circulating tumor cells in women suspected of breast cancer: the BEST (Breast Early Screening Test) study. Asian Pacific Journal of Cancer Prevention 16(5): 1929-1934, 2015

Evaluation of a panel of potential serum tumor markers in women with metastatic breast cancer entered on a prospective chemotherapy clinical trial A North Central cancer treatment group study. Tumor Biology 17(Suppl. 1): 12, 1996

Molecular markers for circulating tumor cells in breast cancer. Journal of Clinical Oncology 29(27_Suppl): 223-223, 2016

Assessment of a six gene panel for the molecular detection of circulating tumor cells in the blood of female cancer patients. Bmc Cancer 10: 666, 2010

Clinical significance of circulating tumor cells in blood by molecular detection and tumor markers in esophageal cancer. Surgery 133(2): 162-169, 2003

Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer. Oncology Letters 13(6): 4627-4632, 2017

Multiple mRNA markers for the detection of circulating tumor cells in breast cancer patients. Genomic Medicine Biomarkers and Health Sciences 4(1-2), 2012

Prognosis of women with stage IV breast cancer depends on detection of circulating tumor cells rather than disseminated tumor cells. Annals of Oncology 19(3): 496-500, 2008

Cancer panel analysis of circulating tumor cells in patients with breast cancer. Oncology Letters 16(1): 612-618, 2018

Evaluation of a novel approach to circulating tumor cell isolation for cancer gene panel analysis in patients with breast cancer. Oncology Letters 13(5): 3025-3031, 2017

Comparison of three molecular assays for the detection and molecular characterization of circulating tumor cells in breast cancer. Breast Cancer Research 15(2): R20, 2013

12 epigenetic markers panel for circulating Dna in plasma in detection of early stage breast cancer. European Journal of Cancer 60: e17-e18, 2016

Circulating miRNAs as surrogate markers for circulating tumor cells and prognostic markers in metastatic breast cancer. Clinical Cancer Research 18(21): 5972-5982, 2012